Enzyme immunochromatography--a quantitative immunoassay requiring no instrumentation. Export

@article{citeulike:2375370, abstract = {We describe a novel test-strip immunoassay for quantifying drugs in biological fluids. This enzyme immunochromatographic ("immunograph") method combines many features of the enzyme-channeling homogeneous immunoassay with immunochromatography and capillary migration to provide a non-separation, non-instrumental assay for theophylline in which quantification is based on the spatial distribution of enzyme label rather than on the modulation of enzyme activity. Sample antigen and hapten-enzyme conjugate are combined and moved by capillary action up a paper strip on which specific antibody has been immobilized. After color development, the assay result is evaluated by measuring the height of the colored zone on the test strip. Quantification is not a function of enzyme activity, so the method is relatively insensitive to sample matrix effects, enzyme instability, temperature, and incubation timing. Either whole blood or plasma can be used as sample. Results correlate well with those by established instrumental methods. The simple, rapid (15 min), two-incubation protocol is well suited for on-site testing in non-laboratory environments.}, author = {Zuk, R. F. and Ginsberg, V. K. and Houts, T. and Rabbie, J. and Merrick, H. and Ullman, E. F. and Fischer, M. M. and Sizto, C. C. and Stiso, S. N. and Litman, D. J.}, citeulike-article-id = {2375370}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/3891138}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=3891138}, citeulike-linkout-2 = {http://www.clinchem.org/cgi/reprint/31/7/1144}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {channeling, chromatography, enzyme, g6pdh, hrp, immunoassay}, month = {July}, number = {7}, pages = {1144--1150}, posted-at = {2008-02-14 16:35:59}, priority = {2}, title = {Enzyme immunochromatography--a quantitative immunoassay requiring no instrumentation.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3891138}, volume = {31}, year = {1985} }

TY - JOUR ID - citeulike:2375370 L3 - citeulike-article-id:2375370 N2 - We describe a novel test-strip immunoassay for quantifying drugs in biological fluids. This enzyme immunochromatographic ("immunograph") method combines many features of the enzyme-channeling homogeneous immunoassay with immunochromatography and capillary migration to provide a non-separation, non-instrumental assay for theophylline in which quantification is based on the spatial distribution of enzyme label rather than on the modulation of enzyme activity. Sample antigen and hapten-enzyme conjugate are combined and moved by capillary action up a paper strip on which specific antibody has been immobilized. After color development, the assay result is evaluated by measuring the height of the colored zone on the test strip. Quantification is not a function of enzyme activity, so the method is relatively insensitive to sample matrix effects, enzyme instability, temperature, and incubation timing. Either whole blood or plasma can be used as sample. Results correlate well with those by established instrumental methods. The simple, rapid (15 min), two-incubation protocol is well suited for on-site testing in non-laboratory environments. IS - 7 JF - Clin Chem SN - 0009-9147 EP - 1150 TI - Enzyme immunochromatography--a quantitative immunoassay requiring no instrumentation. VL - 31 SP - 1144 KW - channeling KW - chromatography KW - enzyme KW - g6pdh KW - hrp KW - immunoassay AU - Zuk, RF AU - Ginsberg, VK AU - Houts, T AU - Rabbie, J AU - Merrick, H AU - Ullman, EF AU - Fischer, MM AU - Sizto, CC AU - Stiso, SN AU - Litman, DJ PY - 1985/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/3891138 ER -