Evaluation of precipitation and direct methods for HDL-cholesterol assay by HPLC. Export

@article{citeulike:2461070, abstract = {HDL-cholesterol (HDL-C) values measured by precipitation (sodium phosphotungstate-MgCl2) and direct methods were compared with those obtained by HPLC with a new column (TSKgel Lipopropak) and an eluent (TSKeluent LP-1). The HDL-C values determined by the precipitation method were significantly (P <0.001) lower than those by the HPLC method, whereas the HDL-C values by the direct method were slightly but significantly higher (P <0.02) than those by the HPLC method. A quantitative HPLC analysis of the cholesterol concentration in HDL and non-HDL fractions in the supernatant of serum separated by precipitation reagents with different MgCl2 concentrations ranging from 7.3 to 44 mmol/L revealed that reagents with >22 mmol/L MgCl2 precipitated part of HDL as well as non-HDL lipoproteins. The HPLC method providing quantitative and qualitative information with high precision was regarded as being a reliable approach for HDL-C assay. The HPLC can be also used to evaluate alternative methods for cholesterol assay.}, address = {Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Chiba, Japan. okazaki.cul@cul.tmd.ac.jp}, author = {Okazaki, M. and Sasamoto, K. and Muramatsu, T. and Hosaki, S.}, citeulike-article-id = {2461070}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9342008}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9342008}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {hdl, hplc, method, mgcl2, reference}, month = {October}, number = {10}, pages = {1885--1890}, posted-at = {2008-03-03 15:50:46}, priority = {2}, title = {Evaluation of precipitation and direct methods for HDL-cholesterol assay by HPLC.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9342008}, volume = {43}, year = {1997} }

TY - JOUR ID - citeulike:2461070 L3 - citeulike-article-id:2461070 N2 - HDL-cholesterol (HDL-C) values measured by precipitation (sodium phosphotungstate-MgCl2) and direct methods were compared with those obtained by HPLC with a new column (TSKgel Lipopropak) and an eluent (TSKeluent LP-1). The HDL-C values determined by the precipitation method were significantly (P <0.001) lower than those by the HPLC method, whereas the HDL-C values by the direct method were slightly but significantly higher (P <0.02) than those by the HPLC method. A quantitative HPLC analysis of the cholesterol concentration in HDL and non-HDL fractions in the supernatant of serum separated by precipitation reagents with different MgCl2 concentrations ranging from 7.3 to 44 mmol/L revealed that reagents with >22 mmol/L MgCl2 precipitated part of HDL as well as non-HDL lipoproteins. The HPLC method providing quantitative and qualitative information with high precision was regarded as being a reliable approach for HDL-C assay. The HPLC can be also used to evaluate alternative methods for cholesterol assay. IS - 10 JF - Clin Chem SN - 0009-9147 AD - Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Chiba, Japan. okazaki.cul@cul.tmd.ac.jp EP - 1890 TI - Evaluation of precipitation and direct methods for HDL-cholesterol assay by HPLC. VL - 43 SP - 1885 KW - hdl KW - hplc KW - method KW - mgcl2 KW - reference AU - Okazaki, M AU - Sasamoto, K AU - Muramatsu, T AU - Hosaki, S PY - 1997/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/9342008 ER -