Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics. Export

@article{citeulike:3090816, abstract = {Tissue microarray (TMA) technology provides a possibility to explore protein expression patterns in a multitude of normal and disease tissues in a high-throughput setting. Although TMAs have been used for analysis of tissue samples, robust methods for studying in vitro cultured cell lines and cell aspirates in a TMA format have been lacking. We have adopted a technique to homogeneously distribute cells in an agarose gel matrix, creating an artificial tissue. This enables simultaneous profiling of protein expression in suspension- and adherent-grown cell samples assembled in a microarray. In addition, the present study provides an optimized strategy for the basic laboratory steps to efficiently produce TMAs. Presented modifications resulted in an improved quality of specimens and a higher section yield compared with standard TMA production protocols. Sections from the generated cell TMAs were tested for immunohistochemical staining properties using 20 well-characterized antibodies. Comparison of immunoreactivity in cultured dispersed cells and corresponding cells in tissue samples showed congruent results for all tested antibodies. We conclude that a modified TMA technique, including cell samples, provides a valuable tool for high-throughput analysis of protein expression, and that this technique can be used for global approaches to explore the human proteome.}, address = {Department of Genetics and Pathology, Rudbeck Laboratory, University Hospital, Uppsala, Sweden.}, author = {Andersson, A. C. and Str\"{o}mberg, S. and B\"{a}ckvall, H. and Kampf, C. and Uhlen, M. and Wester, K. and Pont\'{e}n, F.}, citeulike-article-id = {3090816}, citeulike-linkout-0 = {http://dx.doi.org/10.1369/jhc.6A7001.2006}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16957166}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16957166}, doi = {10.1369/jhc.6A7001.2006}, issn = {0022-1554}, journal = {The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society}, keywords = {cell, microarray}, month = {December}, number = {12}, pages = {1413--1423}, posted-at = {2008-08-06 15:06:08}, priority = {2}, title = {Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics.}, url = {http://dx.doi.org/10.1369/jhc.6A7001.2006}, volume = {54}, year = {2006} }

TY - JOUR ID - citeulike:3090816 L3 - citeulike-article-id:3090816 N2 - Tissue microarray (TMA) technology provides a possibility to explore protein expression patterns in a multitude of normal and disease tissues in a high-throughput setting. Although TMAs have been used for analysis of tissue samples, robust methods for studying in vitro cultured cell lines and cell aspirates in a TMA format have been lacking. We have adopted a technique to homogeneously distribute cells in an agarose gel matrix, creating an artificial tissue. This enables simultaneous profiling of protein expression in suspension- and adherent-grown cell samples assembled in a microarray. In addition, the present study provides an optimized strategy for the basic laboratory steps to efficiently produce TMAs. Presented modifications resulted in an improved quality of specimens and a higher section yield compared with standard TMA production protocols. Sections from the generated cell TMAs were tested for immunohistochemical staining properties using 20 well-characterized antibodies. Comparison of immunoreactivity in cultured dispersed cells and corresponding cells in tissue samples showed congruent results for all tested antibodies. We conclude that a modified TMA technique, including cell samples, provides a valuable tool for high-throughput analysis of protein expression, and that this technique can be used for global approaches to explore the human proteome. IS - 12 JF - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society SN - 0022-1554 AD - Department of Genetics and Pathology, Rudbeck Laboratory, University Hospital, Uppsala, Sweden. EP - 1423 TI - Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics. VL - 54 SP - 1413 KW - cell KW - microarray AU - Andersson, AC AU - Strömberg, S AU - Bäckvall, H AU - Kampf, C AU - Uhlen, M AU - Wester, K AU - Pontén, F PY - 2006/12// UR - http://dx.doi.org/10.1369/jhc.6A7001.2006 ER -