Free cholesterol fiber-optic biosensor for serum samples with simplex optimization. Export

@article{citeulike:4375320, abstract = {An optical fiber biosensor for free cholesterol monitoring in serum samples is described. Silicone-entrapped tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) complex, the luminescence of which is sensitive to oxygen changes, is used as an optical transducer of the oxidation of cholesterol by cholesterol oxidase. The biocatalyst is entrapped in a graphite powder layer deposited onto the dyed silicone film. Optimization of some interdependent chemical variables which affect the performance of the biosensor has been achieved by application of a super-modified simplex method. The dynamic range of the biosensing membranes is found to be 0.15-3.0 mM of free cholesterol. Studies of the reproducibility, stability and interferences of the device, as well as the application of the sensor to measurements in serum samples, are reported. Simplex optimization has proven to be a very useful tool in the search for the optimal conditions for performing analyses with the optical fiber biosensor.}, author = {Marazuela, M. D. and Cuesta, B. and Moreno-Bondi, M. C. and Quejido, A.}, citeulike-article-id = {4375320}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9115690}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9115690}, issn = {0956-5663}, journal = {Biosensors \& bioelectronics}, keywords = {cholesterol, d-o-e, free\_cholesterol, optimisation, simplex}, number = {3}, pages = {233--240}, posted-at = {2009-04-22 14:13:54}, priority = {2}, title = {Free cholesterol fiber-optic biosensor for serum samples with simplex optimization.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9115690}, volume = {12}, year = {1997} }

TY - JOUR ID - citeulike:4375320 L3 - citeulike-article-id:4375320 N2 - An optical fiber biosensor for free cholesterol monitoring in serum samples is described. Silicone-entrapped tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) complex, the luminescence of which is sensitive to oxygen changes, is used as an optical transducer of the oxidation of cholesterol by cholesterol oxidase. The biocatalyst is entrapped in a graphite powder layer deposited onto the dyed silicone film. Optimization of some interdependent chemical variables which affect the performance of the biosensor has been achieved by application of a super-modified simplex method. The dynamic range of the biosensing membranes is found to be 0.15-3.0 mM of free cholesterol. Studies of the reproducibility, stability and interferences of the device, as well as the application of the sensor to measurements in serum samples, are reported. Simplex optimization has proven to be a very useful tool in the search for the optimal conditions for performing analyses with the optical fiber biosensor. IS - 3 JF - Biosensors & bioelectronics SN - 0956-5663 EP - 240 TI - Free cholesterol fiber-optic biosensor for serum samples with simplex optimization. VL - 12 SP - 233 KW - cholesterol KW - d-o-e KW - free_cholesterol KW - optimisation KW - simplex AU - Marazuela, MD AU - Cuesta, B AU - Moreno-Bondi, MC AU - Quejido, A PY - 1997/// UR - http://view.ncbi.nlm.nih.gov/pubmed/9115690 ER -