Determination of [beta]-HCG using amperometric immunosensors based on a conducting immunocomposite Export

@article{citeulike:470484, abstract = {An amperometric immunosensor based on a rigid immunocomposite was built to measure human chorionic gonadotropin [beta]-subunit ([beta]-HCG). The immunocomposite is formed by a conducting graphite-methacrylate matrix containing the anti-[beta]-HCG antibody. The main advantage of this kind of immunosensors is their easy regeneration which can be achieved by a simple polishing of their surface. [beta]-HCG is determined with a sandwich assay using anti-[beta]-HCG conjugate labelled with horseradish peroxidase (HRP). The extent of the immunological interaction is quantified by the activity of the labelling enzyme: peroxidase is regenerated after the enzymatic reduction of H2O2 by hydroquinone which is used as a mediator in the solution. The reduction of hydroquinone is amperometrically monitored using a potential of -0.1 V. Each assay can be realised in less than 40 min. The upper limit corresponds to a [beta]-HCG concentration of 98.0 IU/l and the detection limit to 2.6 IU/l.}, author = {Santandreu, M. and Alegret, S. and F\`{a}bregas, E.}, citeulike-article-id = {470484}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0003-2670(99)00436-5}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(99)00436-5}, citeulike-linkout-2 = {http://www.sciencedirect.com/science/article/B6TF4-3XD3R8V-9/2/e370cface7c8370b9dd98e1513e353df}, day = {20}, doi = {10.1016/S0003-2670(99)00436-5}, journal = {Analytica Chimica Acta}, keywords = {ag\_detection, amperometry, electrochemistry, h\_cg, immunoassay, immunoelectrode, immunosensor, sandwich}, month = {September}, number = {2-3}, pages = {181--188}, posted-at = {2008-12-01 17:40:00}, priority = {2}, title = {Determination of [beta]-HCG using amperometric immunosensors based on a conducting immunocomposite}, url = {http://dx.doi.org/10.1016/S0003-2670(99)00436-5}, volume = {396}, year = {1999} }

TY - JOUR ID - citeulike:470484 L3 - citeulike-article-id:470484 N2 - An amperometric immunosensor based on a rigid immunocomposite was built to measure human chorionic gonadotropin [beta]-subunit ([beta]-HCG). The immunocomposite is formed by a conducting graphite-methacrylate matrix containing the anti-[beta]-HCG antibody. The main advantage of this kind of immunosensors is their easy regeneration which can be achieved by a simple polishing of their surface. [beta]-HCG is determined with a sandwich assay using anti-[beta]-HCG conjugate labelled with horseradish peroxidase (HRP). The extent of the immunological interaction is quantified by the activity of the labelling enzyme: peroxidase is regenerated after the enzymatic reduction of H2O2 by hydroquinone which is used as a mediator in the solution. The reduction of hydroquinone is amperometrically monitored using a potential of -0.1 V. Each assay can be realised in less than 40 min. The upper limit corresponds to a [beta]-HCG concentration of 98.0 IU/l and the detection limit to 2.6 IU/l. IS - 2-3 JF - Analytica Chimica Acta EP - 188 TI - Determination of [beta]-HCG using amperometric immunosensors based on a conducting immunocomposite VL - 396 SP - 181 KW - ag_detection KW - amperometry KW - electrochemistry KW - h_cg KW - immunoassay KW - immunoelectrode KW - immunosensor KW - sandwich AU - Santandreu, M AU - Alegret, S AU - Fàbregas, E PY - 1999/09/20/ UR - http://dx.doi.org/10.1016/S0003-2670(99)00436-5 ER -