A highly sensitive flow-through amperometric immunosensor based on the Peroxidase chip and enzyme-channeling principle. Export

@article{citeulike:562354, abstract = {A concept based on the Peroxidase-chip (P-chip), antibody co-immobilization, competitive and enzyme-channeling principle was exploited to develop an integrated flow-through amperometric biosensor for detection of environmental pollutants such as s-triazine herbicides. In this concept, recombinant peroxidase is immobilized on the gold electrode (P-chip) in such a way that direct electron transfer is achieved. The recognition and quantitation the target analyte is realized through the competition between the simazine-glucose oxidase (GOD) conjugate and free simazine for the binding sites of the monoclonal antibody co-immobilized with peroxidase on the gold electrode. The arrangement allows to generate a specific signal in the presence of glucose through the channeling of H2O2 produced by GOD conjugate bound to the antibody. The immunosensor exhibited 50\% signal decrease (IC50 value) at approximately 0.02 microg l(-1). A concentration of 0.1 ng l(-1) gave a signal clearly distinguishable from the blank whereas the ELISA using the same antibody had a typical detection limit of about 1 microg l(-1), which is four orders of magnitude higher compared to the presented biosensor system. The results demonstrated that gene engineering biomolecules, in this case recombinant peroxidase, might be attractive reagents for the development of electrochemical immunosensors.}, address = {Department of Biotechnology, Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic.}, author = {Zeravik, J. and Ruzgas, T. and Fr\'{a}nek, M.}, citeulike-article-id = {562354}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12896832}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12896832}, day = {1}, issn = {0956-5663}, journal = {Biosens Bioelectron}, keywords = {amperometry, channeling, competition, electrochemistry, fia, immunoelectrode, immunosensor}, month = {October}, number = {11}, pages = {1321--1327}, posted-at = {2008-12-01 17:27:24}, priority = {2}, title = {A highly sensitive flow-through amperometric immunosensor based on the Peroxidase chip and enzyme-channeling principle.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12896832}, volume = {18}, year = {2003} }

TY - JOUR ID - citeulike:562354 L3 - citeulike-article-id:562354 N2 - A concept based on the Peroxidase-chip (P-chip), antibody co-immobilization, competitive and enzyme-channeling principle was exploited to develop an integrated flow-through amperometric biosensor for detection of environmental pollutants such as s-triazine herbicides. In this concept, recombinant peroxidase is immobilized on the gold electrode (P-chip) in such a way that direct electron transfer is achieved. The recognition and quantitation the target analyte is realized through the competition between the simazine-glucose oxidase (GOD) conjugate and free simazine for the binding sites of the monoclonal antibody co-immobilized with peroxidase on the gold electrode. The arrangement allows to generate a specific signal in the presence of glucose through the channeling of H2O2 produced by GOD conjugate bound to the antibody. The immunosensor exhibited 50% signal decrease (IC50 value) at approximately 0.02 microg l(-1). A concentration of 0.1 ng l(-1) gave a signal clearly distinguishable from the blank whereas the ELISA using the same antibody had a typical detection limit of about 1 microg l(-1), which is four orders of magnitude higher compared to the presented biosensor system. The results demonstrated that gene engineering biomolecules, in this case recombinant peroxidase, might be attractive reagents for the development of electrochemical immunosensors. IS - 11 JF - Biosens Bioelectron SN - 0956-5663 AD - Department of Biotechnology, Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic. EP - 1327 TI - A highly sensitive flow-through amperometric immunosensor based on the Peroxidase chip and enzyme-channeling principle. VL - 18 SP - 1321 KW - amperometry KW - channeling KW - competition KW - electrochemistry KW - fia KW - immunoelectrode KW - immunosensor AU - Zeravik, J AU - Ruzgas, T AU - Fránek, M PY - 2003/10/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/12896832 ER -