Competitive solid-phase enzyme immunoassay for measuring digoxin in serum. Export

@article{citeulike:578288, abstract = {In this clinically useful enzyme immunoassay of digoxin in serum, we mix sample, beta-galactosidase-labeled digoxin, and anti-digoxin Fab fragments for 30 min at room temperature, then use Sepharose-bound second antibody for phase separation, and measure the unbound enzyme activity directly in the supernate of the equilibrium reaction mixture. The immunoassay buffer--phosphate-buffered isotonic saline with added rabbit globulin (4 g/L), hydrolyzed gelatin (2 g/L), Brij 96 detergent (5 g/L), glycerol (0.25 mol/L), and N-acetyl-8-anilinonaphthalene-1-sulfonic acid (2 mmol/L)--minimizes serum matrix effects for convenient measuring of unbound enzyme--digoxin conjugate. The immunoassay developed with Fab fragments has better displacement characteristics than that with intact antibody. Performance of the assay compares favorably with that of other manual digoxin immunoassays; in comparison studies with EMIT involving 110 clinical specimens, the coefficient of correlation was 0.97.}, author = {Hinds, J. A. and Pincombe, C. F. and Morris, H. and Duffy, P.}, citeulike-article-id = {578288}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/3510090}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=3510090}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {beads, beta-gal, competition, immunoassay, labeled\_enzyme}, month = {January}, number = {1 Pt 1}, pages = {16--21}, posted-at = {2008-02-11 15:26:03}, priority = {2}, title = {Competitive solid-phase enzyme immunoassay for measuring digoxin in serum.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3510090}, volume = {32}, year = {1986} }

TY - JOUR ID - citeulike:578288 L3 - citeulike-article-id:578288 N2 - In this clinically useful enzyme immunoassay of digoxin in serum, we mix sample, beta-galactosidase-labeled digoxin, and anti-digoxin Fab fragments for 30 min at room temperature, then use Sepharose-bound second antibody for phase separation, and measure the unbound enzyme activity directly in the supernate of the equilibrium reaction mixture. The immunoassay buffer--phosphate-buffered isotonic saline with added rabbit globulin (4 g/L), hydrolyzed gelatin (2 g/L), Brij 96 detergent (5 g/L), glycerol (0.25 mol/L), and N-acetyl-8-anilinonaphthalene-1-sulfonic acid (2 mmol/L)--minimizes serum matrix effects for convenient measuring of unbound enzyme--digoxin conjugate. The immunoassay developed with Fab fragments has better displacement characteristics than that with intact antibody. Performance of the assay compares favorably with that of other manual digoxin immunoassays; in comparison studies with EMIT involving 110 clinical specimens, the coefficient of correlation was 0.97. IS - 1 Pt 1 JF - Clin Chem SN - 0009-9147 EP - 21 TI - Competitive solid-phase enzyme immunoassay for measuring digoxin in serum. VL - 32 SP - 16 KW - beads KW - beta-gal KW - competition KW - immunoassay KW - labeled_enzyme AU - Hinds, JA AU - Pincombe, CF AU - Morris, H AU - Duffy, P PY - 1986/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/3510090 ER -