Sensitive, optimized assay for serum AMP deaminase. Export

@article{citeulike:5890520, abstract = {A sensitive, optimized assay for serum AMP deaminase (EC 3.5.4.6) is presented, which is based on a colorimetric indophenol determination of ammonia liberated during the enzyme reaction. An average enzyme activity in serum of 2.7 U/liter (n = 36, range 0-6.2) for healthy adults was established. Both the enzyme and colorimetric reactions were optimized to give an assay sensitivity of 0.2 U/liter of serum, a 98-99\% analytical recovery in the normal serum enzyme concentration range, and a CV of 7.9\% in-run and 10.6\% between-run. This optimization included studies on specific buffer, pH, ionic strength, and potential activators. The Km for the enzyme in serum was determinated to be 1.4 X 10(-3) mol/liter, which agrees well with the value reported for human skeletal muscle enzyme. Above-normal serum AMP deaminase activity may be present in various myopathies and could be useful in detecting carriers of the X-linked dystrophies.}, author = {Pedersen, R. C. and Berry, A. J.}, citeulike-article-id = {5890520}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/19169}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=19169}, issn = {0009-9147}, journal = {Clinical chemistry}, keywords = {assay, deaminase}, month = {September}, number = {9}, pages = {1726--1733}, posted-at = {2009-10-05 14:47:09}, priority = {2}, title = {Sensitive, optimized assay for serum AMP deaminase.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/19169}, volume = {23}, year = {1977} }

TY - JOUR ID - citeulike:5890520 L3 - citeulike-article-id:5890520 N2 - A sensitive, optimized assay for serum AMP deaminase (EC 3.5.4.6) is presented, which is based on a colorimetric indophenol determination of ammonia liberated during the enzyme reaction. An average enzyme activity in serum of 2.7 U/liter (n = 36, range 0-6.2) for healthy adults was established. Both the enzyme and colorimetric reactions were optimized to give an assay sensitivity of 0.2 U/liter of serum, a 98-99% analytical recovery in the normal serum enzyme concentration range, and a CV of 7.9% in-run and 10.6% between-run. This optimization included studies on specific buffer, pH, ionic strength, and potential activators. The Km for the enzyme in serum was determinated to be 1.4 X 10(-3) mol/liter, which agrees well with the value reported for human skeletal muscle enzyme. Above-normal serum AMP deaminase activity may be present in various myopathies and could be useful in detecting carriers of the X-linked dystrophies. IS - 9 JF - Clinical chemistry SN - 0009-9147 EP - 1733 TI - Sensitive, optimized assay for serum AMP deaminase. VL - 23 SP - 1726 KW - assay KW - deaminase AU - Pedersen, RC AU - Berry, AJ PY - 1977/09// UR - http://view.ncbi.nlm.nih.gov/pubmed/19169 ER -