Immunochemical characterization of commercial protease products. Export

@article{citeulike:5984395, abstract = {Total protease activity at pH 7 and 10.3 of 23 commercial grade enzymes was determined. The type and amount of enzymatic activity varied widely among the products. The wide variation in pH 7.0/pH 10.3 proteolytic activity ratios among products indicated that the products studied contained differing levels of alkaline and neutral proteases.Antisera were prepared against the purified enzyme in detergent grade Enzyme AP, neutral protease from B. megaterium, detergent grade ALK Enzyme, and Thermolysin. The commercial (unpurified) products were classified as neutral subtilopeptidase A and subtilopeptidase B from three Bacillus species using these antisera.It was concluded that standard immunochemical techniques provide rapid and sensitive methods for the preliminary identification of sources and types of proteases present in commercial enzyme products.}, address = {Monsanto Company, St. Louis, Missouri 63166}, author = {Hodson, P. H. and Scharpf, L. G.}, citeulike-article-id = {5984395}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/bit.260150308}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/4198973}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=4198973}, citeulike-linkout-3 = {http://www3.interscience.wiley.com/cgi-bin/abstract/107618432/ABSTRACT}, doi = {10.1002/bit.260150308}, issn = {0006-3592}, journal = {Biotechnology and bioengineering}, keywords = {activity, protease, review}, month = {May}, number = {3}, pages = {519--532}, posted-at = {2009-10-22 11:20:28}, priority = {2}, title = {Immunochemical characterization of commercial protease products.}, url = {http://dx.doi.org/10.1002/bit.260150308}, volume = {15}, year = {1973} }

TY - JOUR ID - citeulike:5984395 L3 - citeulike-article-id:5984395 N2 - Total protease activity at pH 7 and 10.3 of 23 commercial grade enzymes was determined. The type and amount of enzymatic activity varied widely among the products. The wide variation in pH 7.0/pH 10.3 proteolytic activity ratios among products indicated that the products studied contained differing levels of alkaline and neutral proteases.Antisera were prepared against the purified enzyme in detergent grade Enzyme AP, neutral protease from B. megaterium, detergent grade ALK Enzyme, and Thermolysin. The commercial (unpurified) products were classified as neutral subtilopeptidase A and subtilopeptidase B from three Bacillus species using these antisera.It was concluded that standard immunochemical techniques provide rapid and sensitive methods for the preliminary identification of sources and types of proteases present in commercial enzyme products. IS - 3 JF - Biotechnology and bioengineering SN - 0006-3592 AD - Monsanto Company, St. Louis, Missouri 63166 EP - 532 TI - Immunochemical characterization of commercial protease products. VL - 15 SP - 519 KW - activity KW - protease KW - review AU - Hodson, PH AU - Scharpf, LG PY - 1973/05// UR - http://dx.doi.org/10.1002/bit.260150308 ER -