Amperometric enzyme-amplified immunoassays. Export

@article{citeulike:797293, abstract = {Enzyme-amplified immunoassays have been adapted for electrochemical measurement, using an NAD+/NADH redox cycle coupled to an electrode via the active site of diaphorase. Two amperometric methods are described, the first employs an organic conducting salt electrode, NMP+/TCNQ-; the second a platinum wire with ferricyanide as electron transfer mediator. In an immunoenzymometric assay for human prostatic acid phosphatase the sensitivities of the electrochemical methods were comparable to that achieved with the existing optical technique, but the dynamic range of the electrochemical assays was increased by at least two orders of magnitude. It is proposed that electrochemical enzyme-amplified immunoassays may eventually replace their optical counterparts.}, address = {Novo Biolabs Ltd., Downham House, Cambridge, U.K.}, author = {Stanley, C. J. and Cox, R. B. and Cardosi, M. F. and Turner, A. P.}, citeulike-article-id = {797293}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/3047261}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=3047261}, day = {13}, issn = {0022-1759}, journal = {J Immunol Methods}, keywords = {amperometry, amplification, electrochemistry, immunoassay, nadph}, month = {September}, number = {2}, pages = {153--161}, posted-at = {2007-06-06 15:37:16}, priority = {2}, title = {Amperometric enzyme-amplified immunoassays.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3047261}, volume = {112}, year = {1988} }

TY - JOUR ID - citeulike:797293 L3 - citeulike-article-id:797293 N2 - Enzyme-amplified immunoassays have been adapted for electrochemical measurement, using an NAD+/NADH redox cycle coupled to an electrode via the active site of diaphorase. Two amperometric methods are described, the first employs an organic conducting salt electrode, NMP+/TCNQ-; the second a platinum wire with ferricyanide as electron transfer mediator. In an immunoenzymometric assay for human prostatic acid phosphatase the sensitivities of the electrochemical methods were comparable to that achieved with the existing optical technique, but the dynamic range of the electrochemical assays was increased by at least two orders of magnitude. It is proposed that electrochemical enzyme-amplified immunoassays may eventually replace their optical counterparts. IS - 2 JF - J Immunol Methods SN - 0022-1759 AD - Novo Biolabs Ltd., Downham House, Cambridge, U.K. EP - 161 TI - Amperometric enzyme-amplified immunoassays. VL - 112 SP - 153 KW - amperometry KW - amplification KW - electrochemistry KW - immunoassay KW - nadph AU - Stanley, CJ AU - Cox, RB AU - Cardosi, MF AU - Turner, AP PY - 1988/09/13/ UR - http://view.ncbi.nlm.nih.gov/pubmed/3047261 ER -