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biblio24's Tanaka:S [8 articles]

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	Electrochemical immunoassay at a 17beta-estradiol self-assembled monolayer electrode using a redox marker. Analyst, Vol. 128, No. 2. (February 2003), pp. 182-186. by H. Kuramitz, M. Matsuda, J. H. Thomas, K. Sugawara, S. Tanaka posted to ab_detection ag_detection competition electrochemistry immunoassay surface by biblio24 on 2006-10-03 10:29:21 Abstract Copy My Attachments My Copy Abstract A simple electrochemical immunoassay was demonstrated using a 17beta-estradiol modified electrode. 17beta-estradiol was immobilized on the gold electrode surface with a self-assembly technique. The specific binding between estradiol antibody and 17beta-estradiol on the electrode surface was evaluated by monitoring the change in the electrode response with three hydrophilic redox markers. The decrease in the electrode response for the redox marker was observed, when the antibody was bound to the estradiol self-assembled monolayer (SAM) electrode surface. The change in the electrode response ...
	Electrochemical Detection of the Interaction Between Avidin and Biotin Based on the Change of Electrode Response of Copper Enhanced by Biotin Labeled with Thiourea Electroanalysis, Vol. 12, No. 8. (2000), pp. 588-592. by Hideki Kuramitz, Junpei Natsui, Shunitz Tanaka, Kiyoshi Hasebe posted to electrochemistry immunoassay mediator_labeled by biblio24 on 2006-10-03 10:27:16 Abstract Copy My Attachments My Copy Abstract A new avidin-biotin assay based on monitoring the change in the electrode response of the copper enhanced by the labeled biotin was investigated. The biotin labeled with thiourea was prepared as a bifunctional compound. The labeled biotin exhibited a high enhancement effect toward the anodic stripping wave of copper as well as a specifical binding with avidin. The anodic stripping wave of copper decreased when the labeled biotin bound with avidin. The calibration plot for avidin was linear between 1.0×10-8 mol/L and ...
	Voltammetric detection of lectin using sugar labeled with electroactive substance. Analytical Sciences, Vol. 17, No. 1. (January 2001), pp. 21-25. by K. Sugawara, H. Kuramitz, T. Kaneko, S. Hoshi, K. Akatsuka, S. Tanaka posted to ag_detection competition homogeneous immunoassay immunosensor mediator_labeled voltametry by biblio24 on 2006-08-17 11:26:22 Abstract Copy My Attachments My Copy Abstract The voltammetric detection of soybean agglutinin (SBA) was investigated on the basis of an interaction between the lectin and a sugar. Because galactose and lactose combined with SBA, the sugars were labeled by a Schiff base with an electroactive daunomycin. After the labeled sugar and SBA were mixed, measurements were carried out by voltammetry. When SBA-sugar binding occurs, a part of daunomycin of the labeled sugar is taken to the binding sites. As a result, SBA is detected by a change ...
	Electrochemical Assay of Avidin and Biotin Using a Biotin Derivative Labeled with an Electroactive Compound Analytical Chemistry, Vol. 67, No. 2. (1995), pp. 299-302. by Kazuharu Sugawara, Shunitz Tanaka, Hiroshi Nakamura posted to ag_detection competition electrochemistry homogeneous immunoassay immunosensor mediator_labeled by biblio24 on 2006-08-17 11:26:22 Abstract Copy My Attachments My Copy Abstract A new avidin-biotin assay was developed based on electrochemical detection. The assay is achieved by biotin labeled with an electroactive compound. That is, because avidin-biotin binding makes a labeled biotin electroinactive, avidin-biotin interaction can be detected indirectly by a change in the electrode response of the labeled biotin. Daunomycin was selected as an electroactive labeling reagent for biotin, and labeled biotin was prepared to cany out the electrochemical assay. By the addition of avidin, the electrode response of the labeled biotin ...
	Electrochemical evaluation of the interaction between endocrine disrupter chemicals and estrogen receptor using 17,beta-estradiol labeled with daunomycin. Analytical Chemistry, Vol. 74, No. 3. (February 2002), pp. 533-538. by Hideki Kuramitz, Junpei Natsui, Kazuharu Sugawara, Shinji Itoh, Shunitz Tanaka posted to ag_detection competition electrochemistry homogeneous immunoassay immunosensor mediator_labeled by biblio24 on 2006-08-17 11:26:22 Abstract Copy My Attachments My Copy Abstract A new electrochemical screening method for endocrine disrupting chemicals (EDCs) was developed. To evaluate the binding capacity of EDCs to the estrogen receptor (ER), 17beta-estradiol labeled with daunomycin as an electroactive compound was prepared. The electrochemical sensitivity of the prepared labeled estradiol (LE) was high, as compared with daunomycin. The interaction between LE and ER was observed by the decrease in the electrode response of LE, indicating the specific binding of LE with ER. The competitive reaction between LE and 17beta-estradiol ...
	Electrochemical Detection of the Interaction Between Avidin and Biotin Based on the Change of Electrode Response of Copper Enhanced by Biotin Labeled with Thiourea Electroanalysis, Vol. 12, No. 8. (May 2000), pp. 588-592. by Hideki Kuramitz, Junpei Natsui, Shunitz Tanaka, Kiyoshi Hasebe posted to ag_detection competition electrochemistry homogeneous mediator_labeled by biblio24 on 2006-08-17 11:26:22 Abstract Copy My Attachments My Copy Abstract A new avidin-biotin assay based on monitoring the change in the electrode response of the copper enhanced by the labeled biotin was investigated. The biotin labeled with thiourea was prepared as a bifunctional compound. The labeled biotin exhibited a high enhancement effect toward the anodic stripping wave of copper as well as a specifical binding with avidin. The anodic stripping wave of copper decreased when the labeled biotin bound with avidin. The calibration plot for avidin was linear between 1.0×10-8 mol/L ...
	Evaluation of Binding Between Electroactive Biotin Derivative and Streptavidin Immobilized on Chitin Film Electroanalysis, Vol. 17, No. 18. (September 2005), pp. 1659-1664. by Kazuharu Sugawara, George Hirabayashi, Naoto Kamiya, Hideki Kuramitz, Shunitz Tanaka posted to ag_detection competition electrochemistry homogeneous mediator_labeled by biblio24 on 2006-08-17 11:26:22 Abstract Copy My Attachments My Copy Abstract Evaluation of the streptavidin-biotin binding at the surface of chitin film was carried out with voltammetry. Immobilization of streptavidin was attempted to the protonated chitin film, based on an electrostatic interaction that hardly causes any change in the protein structure. The streptavidin-biotin binding was estimated from changes in the electrode response of biotin labeled with an electroactive compound. Although the response of daunomycin as an electroactive compound did not change at an electrode covered with streptavidin/chitin film, the response of the ...
	Electrochemical immunoassay at a 17beta-estradiol self-assembled monolayer electrode using a redox marker. Analyst, Vol. 128, No. 2. (February 2003), pp. 182-186. by H. Kuramitz, M. Matsuda, J. H. Thomas, K. Sugawara, S. Tanaka posted to ab_detection ag_detection electrochemistry immunoassay by biblio24 on 2006-08-17 11:26:17 Abstract Copy My Attachments My Copy Abstract A simple electrochemical immunoassay was demonstrated using a 17beta-estradiol modified electrode. 17beta-estradiol was immobilized on the gold electrode surface with a self-assembly technique. The specific binding between estradiol antibody and 17beta-estradiol on the electrode surface was evaluated by monitoring the change in the electrode response with three hydrophilic redox markers. The decrease in the electrode response for the redox marker was observed, when the antibody was bound to the estradiol self-assembled monolayer (SAM) electrode surface. The change in the electrode response ...

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