OsMADS51 Is a Short-Day Flowering Promoter That Functions Upstream of Ehd1, OsMADS14, and Hd3a Export

@article{citeulike:2192244, abstract = {Although flowering regulatory mechanisms have been extensively studied in Arabidopsis (Arabidopsis thaliana), those in other species have not been well elucidated. Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa). In SDs OsMADS51 null mutants flowered 2 weeks later than normal, whereas in long days loss of OsMADS51 had little effect on flowering. Transcript levels of three flowering regulatorsEhd1, OsMADS14, and Hd3awere decreased in these mutants, whereas those of OsGI and Hd1 were unchanged. Ectopic expression of OsMADS51 caused flowering to occur about 7 d earlier only in SDs. In ectopic expression lines, transcript levels of Ehd1, OsMADS14, and Hd3a were increased, but those of OsGI and Hd1 remained the same. These results indicate that OsMADS51 is a flowering promoter, particularly in SDs, and that this gene functions upstream of Ehd1, OsMADS14, and Hd3a. To further investigate the relationship with other flowering promoters, we generated transgenic plants in which expression of Ehd1 or OsGI was suppressed. In Ehd1 RNA interference plants, OsMADS51 expression was not affected, supporting our conclusion that the MADS-box gene functions upstream of Ehd1. However, in OsGI antisense plants, the OsMADS51 transcript level was reduced. In addition, the circadian expression pattern for this MADS-box gene was similar to that for OsGI. These results demonstrate that OsMADS51 functions downstream of OsGI. In summary, OsMADS51 is a novel flowering promoter that transmits a SD promotion signal from OsGI to Ehd1. 10.1104/pp.107.103291}, author = {Kim, Song L. and Lee, Shinyoung and Kim, Hyo J. and Nam, Hong G. and An, Gynheung}, citeulike-article-id = {2192244}, citeulike-linkout-0 = {http://dx.doi.org/10.1104/pp.107.103291}, citeulike-linkout-1 = {http://www.plantphysiol.org/cgi/content/abstract/145/4/1484}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17951465}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17951465}, day = {1}, doi = {10.1104/pp.107.103291}, journal = {Plant Physiol.}, keywords = {floral, ft, oryza, transition}, month = {December}, number = {4}, pages = {1484--1494}, posted-at = {2008-01-03 20:40:20}, priority = {3}, title = {OsMADS51 Is a Short-Day Flowering Promoter That Functions Upstream of Ehd1, OsMADS14, and Hd3a}, url = {http://dx.doi.org/10.1104/pp.107.103291}, volume = {145}, year = {2007} }

TY - JOUR ID - citeulike:2192244 L3 - citeulike-article-id:2192244 N2 - Although flowering regulatory mechanisms have been extensively studied in Arabidopsis (Arabidopsis thaliana), those in other species have not been well elucidated. Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa). In SDs OsMADS51 null mutants flowered 2 weeks later than normal, whereas in long days loss of OsMADS51 had little effect on flowering. Transcript levels of three flowering regulatorsEhd1, OsMADS14, and Hd3awere decreased in these mutants, whereas those of OsGI and Hd1 were unchanged. Ectopic expression of OsMADS51 caused flowering to occur about 7 d earlier only in SDs. In ectopic expression lines, transcript levels of Ehd1, OsMADS14, and Hd3a were increased, but those of OsGI and Hd1 remained the same. These results indicate that OsMADS51 is a flowering promoter, particularly in SDs, and that this gene functions upstream of Ehd1, OsMADS14, and Hd3a. To further investigate the relationship with other flowering promoters, we generated transgenic plants in which expression of Ehd1 or OsGI was suppressed. In Ehd1 RNA interference plants, OsMADS51 expression was not affected, supporting our conclusion that the MADS-box gene functions upstream of Ehd1. However, in OsGI antisense plants, the OsMADS51 transcript level was reduced. In addition, the circadian expression pattern for this MADS-box gene was similar to that for OsGI. These results demonstrate that OsMADS51 functions downstream of OsGI. In summary, OsMADS51 is a novel flowering promoter that transmits a SD promotion signal from OsGI to Ehd1. 10.1104/pp.107.103291 IS - 4 JF - Plant Physiol. EP - 1494 TI - OsMADS51 Is a Short-Day Flowering Promoter That Functions Upstream of Ehd1, OsMADS14, and Hd3a VL - 145 SP - 1484 KW - floral KW - ft KW - oryza KW - transition AU - Kim, Song AU - Lee, Shinyoung AU - Kim, Hyo AU - Nam, Hong AU - An, Gynheung PY - 2007/12/01/ UR - http://dx.doi.org/10.1104/pp.107.103291 ER -