The Cold-Inducible CBF1 Factor-Dependent Signaling Pathway Modulates the Accumulation of the Growth-Repressing DELLA Proteins via Its Effect on Gibberellin Metabolism Export

@article{citeulike:3345692, abstract = {Plants have evolved robust mechanisms to respond and adapt to unfavorable environmental conditions, such as low temperature. The C-repeat/drought-responsive element binding factor CBF1/DREB1b gene encodes a transcriptional activator transiently induced by cold that controls the expression of a set of genes responding to low temperature (the CBF regulon). Constitutive expression of CBF1 confers freezing tolerance but also slows growth. Here, we propose that low temperature-induced CBF1 expression restrains growth at least in part by allowing the accumulation of DELLAs, a family of nuclear growth-repressing proteins, the degradation of which is stimulated by gibberellin (GA). We show that cold/CBF1 enhances the accumulation of a green fluorescent protein (GFP)-tagged DELLA protein (GFP-RGA) by reducing GA content through stimulating expression of GA-inactivating GA 2-oxidase genes. Accordingly, transgenic plants that constitutively express CBF1 accumulate less bioactive GA and as a consequence exhibit dwarfism and late flowering. Both phenotypes are suppressed when CBF1 is expressed in a line lacking two DELLA proteins, GA-INSENSITIVE and REPRESSOR OF GA1-3. In addition, we show that DELLAs contribute significantly to CBF1-induced cold acclimation and freezing tolerance by a mechanism that is distinct from the CBF regulon. We conclude that DELLAs are components of the CBF1-mediated cold stress response. 10.1105/tpc.108.058941}, author = {Achard, Patrick and Gong, Fan and Cheminant, Soizic and Alioua, Malek and Hedden, Peter and Genschik, Pascal}, citeulike-article-id = {3345692}, citeulike-linkout-0 = {http://dx.doi.org/10.1105/tpc.108.058941}, citeulike-linkout-1 = {http://www.plantcell.org/cgi/content/abstract/20/8/2117}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18757556}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18757556}, day = {29}, doi = {10.1105/tpc.108.058941}, journal = {Plant Cell}, keywords = {cold, della, dreb, gibberellin}, month = {August}, pages = {tpc.108.058941+}, posted-at = {2008-09-27 23:23:22}, priority = {2}, title = {The Cold-Inducible CBF1 Factor-Dependent Signaling Pathway Modulates the Accumulation of the Growth-Repressing DELLA Proteins via Its Effect on Gibberellin Metabolism}, url = {http://dx.doi.org/10.1105/tpc.108.058941}, year = {2008} }

TY - JOUR ID - citeulike:3345692 L3 - citeulike-article-id:3345692 N2 - Plants have evolved robust mechanisms to respond and adapt to unfavorable environmental conditions, such as low temperature. The C-repeat/drought-responsive element binding factor CBF1/DREB1b gene encodes a transcriptional activator transiently induced by cold that controls the expression of a set of genes responding to low temperature (the CBF regulon). Constitutive expression of CBF1 confers freezing tolerance but also slows growth. Here, we propose that low temperature-induced CBF1 expression restrains growth at least in part by allowing the accumulation of DELLAs, a family of nuclear growth-repressing proteins, the degradation of which is stimulated by gibberellin (GA). We show that cold/CBF1 enhances the accumulation of a green fluorescent protein (GFP)-tagged DELLA protein (GFP-RGA) by reducing GA content through stimulating expression of GA-inactivating GA 2-oxidase genes. Accordingly, transgenic plants that constitutively express CBF1 accumulate less bioactive GA and as a consequence exhibit dwarfism and late flowering. Both phenotypes are suppressed when CBF1 is expressed in a line lacking two DELLA proteins, GA-INSENSITIVE and REPRESSOR OF GA1-3. In addition, we show that DELLAs contribute significantly to CBF1-induced cold acclimation and freezing tolerance by a mechanism that is distinct from the CBF regulon. We conclude that DELLAs are components of the CBF1-mediated cold stress response. 10.1105/tpc.108.058941 JF - Plant Cell TI - The Cold-Inducible CBF1 Factor-Dependent Signaling Pathway Modulates the Accumulation of the Growth-Repressing DELLA Proteins via Its Effect on Gibberellin Metabolism SP - tpc.108.058941 KW - cold KW - della KW - dreb KW - gibberellin AU - Achard, Patrick AU - Gong, Fan AU - Cheminant, Soizic AU - Alioua, Malek AU - Hedden, Peter AU - Genschik, Pascal PY - 2008/08/29/ UR - http://dx.doi.org/10.1105/tpc.108.058941 ER -