Physical Morphology and Surface Properties of Unsaturated Pseudomonas putida Biofilms Export

@article{citeulike:4021657, abstract = {Unsaturated biofilms of Pseudomonas putida, i.e., biofilms grown in humid air, were analyzed by atomic force microscopy to determine surface morphology, roughness, and adhesion forces in the outer and basal cell layers of fresh and desiccated biofilms. Desiccated biofilms were equilibrated with a 75.5\% relative humidity atmosphere, which is far below the relative humidity of 98 to 99\% at which these biofilms were cultured. In sharp contrast to the effects of drying on biofilms grown in fluid, we observed that drying caused little change in morphology, roughness, or adhesion forces in these unsaturated biofilms. Surface roughness for moist and dry biofilms increased approximately linearly with increasing scan sizes. This indicated that the divides between bacteria contributed more to overall roughness than did extracellular polymeric substances (EPS) on individual bacteria. The EPS formed higher-order structures we termed mesostructures. These mesostructures are much larger than the discrete polymers of glycolipids and proteins that have been previously characterized on the outer surface of these gram-negative bacteria. 10.1128/JB.182.13.3809-3815.2000}, author = {Auerbach, Ilene D. and Sorensen, Cody and Hansma, Helen G. and Holden, Patricia A.}, citeulike-article-id = {4021657}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JB.182.13.3809-3815.2000}, citeulike-linkout-1 = {http://jb.asm.org/cgi/content/abstract/182/13/3809}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/10850998}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=10850998}, day = {1}, doi = {10.1128/JB.182.13.3809-3815.2000}, journal = {J. Bacteriol.}, keywords = {afm, air, biofilm}, month = {July}, number = {13}, pages = {3809--3815}, posted-at = {2009-02-07 13:28:36}, priority = {2}, title = {Physical Morphology and Surface Properties of Unsaturated Pseudomonas putida Biofilms}, url = {http://dx.doi.org/10.1128/JB.182.13.3809-3815.2000}, volume = {182}, year = {2000} }

TY - JOUR ID - citeulike:4021657 L3 - citeulike-article-id:4021657 N2 - Unsaturated biofilms of Pseudomonas putida, i.e., biofilms grown in humid air, were analyzed by atomic force microscopy to determine surface morphology, roughness, and adhesion forces in the outer and basal cell layers of fresh and desiccated biofilms. Desiccated biofilms were equilibrated with a 75.5% relative humidity atmosphere, which is far below the relative humidity of 98 to 99% at which these biofilms were cultured. In sharp contrast to the effects of drying on biofilms grown in fluid, we observed that drying caused little change in morphology, roughness, or adhesion forces in these unsaturated biofilms. Surface roughness for moist and dry biofilms increased approximately linearly with increasing scan sizes. This indicated that the divides between bacteria contributed more to overall roughness than did extracellular polymeric substances (EPS) on individual bacteria. The EPS formed higher-order structures we termed mesostructures. These mesostructures are much larger than the discrete polymers of glycolipids and proteins that have been previously characterized on the outer surface of these gram-negative bacteria. 10.1128/JB.182.13.3809-3815.2000 IS - 13 JF - J. Bacteriol. EP - 3815 TI - Physical Morphology and Surface Properties of Unsaturated Pseudomonas putida Biofilms VL - 182 SP - 3809 KW - afm KW - air KW - biofilm AU - Auerbach, Ilene AU - Sorensen, Cody AU - Hansma, Helen AU - Holden, Patricia PY - 2000/07/01/ UR - http://dx.doi.org/10.1128/JB.182.13.3809-3815.2000 ER -