Scanning ion conductance microscopy of living cells. Export

@article{citeulike:4071758, abstract = {Currently there is a great interest in using scanning probe microscopy to study living cells. However, in most cases the contact the probe makes with the soft surface of the cell deforms or damages it. Here we report a scanning ion conductance microscope specially developed for imaging living cells. A key feature of the instrument is its scanning algorithm, which maintains the working distance between the probe and the sample such that they do not make direct physical contact with each other. Numerical simulation of the probe/sample interaction, which closely matches the experimental observations, provides the optimum working distance. The microscope scans highly convoluted surface structures without damaging them and reveals the true topography of cell surfaces. The images resemble those produced by scanning electron microscopy, with the significant difference that the cells remain viable and active. The instrument can monitor small-scale dynamics of cell surfaces as well as whole-cell movement.}, author = {Korchev, Y. E. and Bashford, C. L. and Milovanovic, M. and Vodyanoy, I. and Lab, M. J.}, citeulike-article-id = {4071758}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0006-3495(97)78100-1}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/9251784}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=9251784}, doi = {10.1016/S0006-3495(97)78100-1}, issn = {0006-3495}, journal = {Biophysical journal}, keywords = {cells, sicm}, month = {August}, number = {2}, pages = {653--658}, posted-at = {2009-02-19 17:25:49}, priority = {2}, title = {Scanning ion conductance microscopy of living cells.}, url = {http://dx.doi.org/10.1016/S0006-3495(97)78100-1}, volume = {73}, year = {1997} }

TY - JOUR ID - citeulike:4071758 L3 - citeulike-article-id:4071758 N2 - Currently there is a great interest in using scanning probe microscopy to study living cells. However, in most cases the contact the probe makes with the soft surface of the cell deforms or damages it. Here we report a scanning ion conductance microscope specially developed for imaging living cells. A key feature of the instrument is its scanning algorithm, which maintains the working distance between the probe and the sample such that they do not make direct physical contact with each other. Numerical simulation of the probe/sample interaction, which closely matches the experimental observations, provides the optimum working distance. The microscope scans highly convoluted surface structures without damaging them and reveals the true topography of cell surfaces. The images resemble those produced by scanning electron microscopy, with the significant difference that the cells remain viable and active. The instrument can monitor small-scale dynamics of cell surfaces as well as whole-cell movement. IS - 2 JF - Biophysical journal SN - 0006-3495 EP - 658 TI - Scanning ion conductance microscopy of living cells. VL - 73 SP - 653 KW - cells KW - sicm AU - Korchev, YE AU - Bashford, CL AU - Milovanovic, M AU - Vodyanoy, I AU - Lab, MJ PY - 1997/08// UR - http://dx.doi.org/10.1016/S0006-3495(97)78100-1 ER -