In Vivo Imaging of S-Layer Nanoarrays on Corynebacterium glutamicum Export

@article{citeulike:5447107, abstract = {PMID: 19642621 Crystalline bacterial cell surface layers (S-layers) are monomolecular arrays of (glyco)proteins that have recently produced a wealth of new opportunities in nanotechnology. Whereas the in vitro imaging of isolated S-layers is well established, their direct imaging on live cells remains very challenging. Here we use atomic force microscopy (AFM) to visualize S-layer nanoarrays on living Corynebacterium glutamicum bacteria. We demonstrate the presence of two highly ordered surface layers. The most external layer represents the hexagonal S-layer, and the inner layer displays regular patterns of nanogrooves that could act as a biomolecular template promoting the 2-D assembly of S-layer monomers. These nanoscale analyses open new avenues for understanding the structure of protein monomolecular arrays, which is a crucial challenge in current nanoscience and life science research.}, author = {Dupres, Vincent and Alsteens, David and Pauwels, Kristof and Dufrêne, Yves F.}, citeulike-article-id = {5447107}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/la902238q}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/la902238q}, doi = {10.1021/la902238q}, journal = {Langmuir}, keywords = {afm, corynebacterium, glutamicum, pore, trapping}, number = {0}, posted-at = {2009-08-17 13:22:01}, priority = {2}, title = {In Vivo Imaging of S-Layer Nanoarrays on Corynebacterium glutamicum}, url = {http://dx.doi.org/10.1021/la902238q}, volume = {0}, year = {0000} }

TY - JOUR ID - citeulike:5447107 L3 - citeulike-article-id:5447107 N2 - PMID: 19642621 Crystalline bacterial cell surface layers (S-layers) are monomolecular arrays of (glyco)proteins that have recently produced a wealth of new opportunities in nanotechnology. Whereas the in vitro imaging of isolated S-layers is well established, their direct imaging on live cells remains very challenging. Here we use atomic force microscopy (AFM) to visualize S-layer nanoarrays on living Corynebacterium glutamicum bacteria. We demonstrate the presence of two highly ordered surface layers. The most external layer represents the hexagonal S-layer, and the inner layer displays regular patterns of nanogrooves that could act as a biomolecular template promoting the 2-D assembly of S-layer monomers. These nanoscale analyses open new avenues for understanding the structure of protein monomolecular arrays, which is a crucial challenge in current nanoscience and life science research. IS - 0 JF - Langmuir TI - In Vivo Imaging of S-Layer Nanoarrays on Corynebacterium glutamicum VL - 0 KW - afm KW - corynebacterium KW - glutamicum KW - pore KW - trapping AU - Dupres, Vincent AU - Alsteens, David AU - Pauwels, Kristof AU - Dufrêne, Yves PY - 2000/// UR - http://dx.doi.org/10.1021/la902238q ER -