Improvement of the pore trapping method to immobilize vital coccoid bacteria for high-resolution AFM: a study of <i>Staphylococcus aureus</i> Export

@article{citeulike:6112747, abstract = {Preparation of vital bacteria for atomic force microscope study under aqueous fluid, such as physiological buffer or bacterial growth medium, presents challenges as cells will often desorb from the supporting surface or be dislodged by the atomic force microscope tip during imaging. An established method of immobilizing coccoid bacteria is to trap cells in polycarbonate track etched filter pores. We have significantly improved this method by modifying the pore diameter of commercially available filters to correspond to the diameter of the target strain, enabling high-resolution imaging of stationary organisms under buffer and dividing organisms under growth media.}, address = {School of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, U.K.; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K.; Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds LS2 9LU, U.K.}, author = {Turner, R. D. and Thomson, N. H. and Kirkham, J. and Devine, D.}, citeulike-article-id = {6112747}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1365-2818.2009.03333.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/122659373/ABSTRACT}, doi = {10.1111/j.1365-2818.2009.03333.x}, issn = {1365-2818}, journal = {Journal of Microscopy}, keywords = {afm, aureus, immobilisation, staphylococcus}, number = {9999}, posted-at = {2009-11-15 12:00:32}, priority = {2}, title = {Improvement of the pore trapping method to immobilize vital coccoid bacteria for high-resolution AFM: a study of <i>Staphylococcus aureus</i>}, url = {http://dx.doi.org/10.1111/j.1365-2818.2009.03333.x}, volume = {9999}, year = {2009} }

TY - JOUR ID - citeulike:6112747 L3 - citeulike-article-id:6112747 N2 - Preparation of vital bacteria for atomic force microscope study under aqueous fluid, such as physiological buffer or bacterial growth medium, presents challenges as cells will often desorb from the supporting surface or be dislodged by the atomic force microscope tip during imaging. An established method of immobilizing coccoid bacteria is to trap cells in polycarbonate track etched filter pores. We have significantly improved this method by modifying the pore diameter of commercially available filters to correspond to the diameter of the target strain, enabling high-resolution imaging of stationary organisms under buffer and dividing organisms under growth media. IS - 9999 JF - Journal of Microscopy SN - 1365-2818 AD - School of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, U.K.; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K.; Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds LS2 9LU, U.K. TI - Improvement of the pore trapping method to immobilize vital coccoid bacteria for high-resolution AFM: a study of <i>Staphylococcus aureus</i> VL - 9999 KW - afm KW - aureus KW - immobilisation KW - staphylococcus AU - Turner, RD AU - Thomson, NH AU - Kirkham, J AU - Devine, D PY - 2009/// UR - http://dx.doi.org/10.1111/j.1365-2818.2009.03333.x ER -