Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission Export

@article{citeulike:911215, abstract = {The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken. This is accomplished by quenching excited organic molecules at the rim of the focal spot through stimulated emission. Along the optic axis, the spot size was reduced by up to 6 times beyond the diffraction barrier. The simultaneous 2-fold improvement in the radial direction rendered a nearly spherical fluorescence spot with a diameter of 90\^{a}€“110 nm. The spot volume of down to 0.67 attoliters is 18 times smaller than that of confocal microscopy, thus making our results also relevant to three-dimensional photochemistry and single molecule spectroscopy. Images of live cells reveal greater details.}, address = {Max-Planck-Institute for Biophysical Chemistry, High Resolution Optical Microscopy Group, 37070 G\"{o}ttingen, Germany.}, author = {Klar, Thomas A. and Jakobs, Stefan and Dyba, Marcus and Egner, Alexander and Hell, Stefan W.}, citeulike-article-id = {911215}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.97.15.8206}, citeulike-linkout-1 = {http://www.pnas.org/content/97/15/8206.full.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/97/15/8206.full.full.pdf}, citeulike-linkout-3 = {http://www.pnas.org/cgi/content/abstract/97/15/8206}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/10899992}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=10899992}, day = {18}, doi = {10.1073/pnas.97.15.8206}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {bacteria, sted, thesis}, month = {July}, number = {15}, pages = {8206--8210}, posted-at = {2009-02-10 11:23:28}, priority = {2}, title = {Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission}, url = {http://dx.doi.org/10.1073/pnas.97.15.8206}, volume = {97}, year = {2000} }

TY - JOUR ID - citeulike:911215 L3 - citeulike-article-id:911215 N2 - The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken. This is accomplished by quenching excited organic molecules at the rim of the focal spot through stimulated emission. Along the optic axis, the spot size was reduced by up to 6 times beyond the diffraction barrier. The simultaneous 2-fold improvement in the radial direction rendered a nearly spherical fluorescence spot with a diameter of 90–110 nm. The spot volume of down to 0.67 attoliters is 18 times smaller than that of confocal microscopy, thus making our results also relevant to three-dimensional photochemistry and single molecule spectroscopy. Images of live cells reveal greater details. IS - 15 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Max-Planck-Institute for Biophysical Chemistry, High Resolution Optical Microscopy Group, 37070 Göttingen, Germany. EP - 8210 TI - Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission VL - 97 SP - 8206 KW - bacteria KW - sted KW - thesis AU - Klar, Thomas AU - Jakobs, Stefan AU - Dyba, Marcus AU - Egner, Alexander AU - Hell, Stefan PY - 2000/07/18/ UR - http://dx.doi.org/10.1073/pnas.97.15.8206 ER -