Adipose tissue interleukin-18 mRNA and plasma interleukin-18: effect of obesity and exercise. Export

@article{citeulike:2961392, abstract = {OBJECTIVES: Obesity and a physically inactive lifestyle are associated with increased risk of developing insulin resistance. The hypothesis that obesity is associated with increased adipose tissue (AT) interleukin (IL)-18 mRNA expression and that AT IL-18 mRNA expression is related to insulin resistance was tested. Furthermore, we speculated that acute exercise and exercise training would regulate AT IL-18 mRNA expression. RESEARCH METHODS AND PROCEDURES: Non-obese subjects with BMI < 30 kg/m(2) (women: n = 18; men; n = 11) and obese subjects with BMI >30 kg/m(2) (women: n = 6; men: n = 7) participated in the study. Blood samples and abdominal subcutaneous AT biopsies were obtained at rest, immediately after an acute exercise bout, and at 2 hours or 10 hours of recovery. After 8 weeks of exercise training of the obese group, sampling was repeated 48 hours after the last training session. RESULTS: AT IL-18 mRNA content and plasma IL-18 concentration were higher (p < 0.05) in the obese group than in the non-obese group. AT IL-18 mRNA content and plasma IL-18 concentration was positively correlated (p < 0.05) with insulin resistance. While acute exercise did not affect IL-18 mRNA expression at the studied time-points, exercise training reduced AT IL-18 mRNA content by 20\% in both sexes. DISCUSSION: Because obesity and insulin resistance were associated with elevated AT IL-18 mRNA and plasma IL-18 levels, the training-induced lowering of AT IL-18 mRNA content may contribute to the beneficial effects of regular physical activity with improved insulin sensitivity.}, address = {Centre of Inflammation and Metabolism, Department of Molecular Biology, The August Krogh Building, University of Copenhagen, Copenhagen, Denmark. LLeick@aki.ku.dk}, author = {Leick, L. and Lindegaard, B. and Stensvold, D. and Plomgaard, P. and Saltin, B. and Pilegaard, H.}, citeulike-article-id = {2961392}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/17299108}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=17299108}, issn = {1930-7381}, journal = {Obesity (Silver Spring, Md.)}, keywords = {adipose-tissue, bmi, il-18}, month = {February}, number = {2}, pages = {356--363}, posted-at = {2008-07-03 22:41:26}, priority = {2}, title = {Adipose tissue interleukin-18 mRNA and plasma interleukin-18: effect of obesity and exercise.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17299108}, volume = {15}, year = {2007} }

TY - JOUR ID - citeulike:2961392 L3 - citeulike-article-id:2961392 N2 - OBJECTIVES: Obesity and a physically inactive lifestyle are associated with increased risk of developing insulin resistance. The hypothesis that obesity is associated with increased adipose tissue (AT) interleukin (IL)-18 mRNA expression and that AT IL-18 mRNA expression is related to insulin resistance was tested. Furthermore, we speculated that acute exercise and exercise training would regulate AT IL-18 mRNA expression. RESEARCH METHODS AND PROCEDURES: Non-obese subjects with BMI < 30 kg/m(2) (women: n = 18; men; n = 11) and obese subjects with BMI >30 kg/m(2) (women: n = 6; men: n = 7) participated in the study. Blood samples and abdominal subcutaneous AT biopsies were obtained at rest, immediately after an acute exercise bout, and at 2 hours or 10 hours of recovery. After 8 weeks of exercise training of the obese group, sampling was repeated 48 hours after the last training session. RESULTS: AT IL-18 mRNA content and plasma IL-18 concentration were higher (p < 0.05) in the obese group than in the non-obese group. AT IL-18 mRNA content and plasma IL-18 concentration was positively correlated (p < 0.05) with insulin resistance. While acute exercise did not affect IL-18 mRNA expression at the studied time-points, exercise training reduced AT IL-18 mRNA content by 20% in both sexes. DISCUSSION: Because obesity and insulin resistance were associated with elevated AT IL-18 mRNA and plasma IL-18 levels, the training-induced lowering of AT IL-18 mRNA content may contribute to the beneficial effects of regular physical activity with improved insulin sensitivity. IS - 2 JF - Obesity (Silver Spring, Md.) SN - 1930-7381 AD - Centre of Inflammation and Metabolism, Department of Molecular Biology, The August Krogh Building, University of Copenhagen, Copenhagen, Denmark. LLeick@aki.ku.dk EP - 363 TI - Adipose tissue interleukin-18 mRNA and plasma interleukin-18: effect of obesity and exercise. VL - 15 SP - 356 KW - adipose-tissue KW - bmi KW - il-18 AU - Leick, L AU - Lindegaard, B AU - Stensvold, D AU - Plomgaard, P AU - Saltin, B AU - Pilegaard, H PY - 2007/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/17299108 ER -