Predictive value of interleukin-10 promoter genotypes and haplotypes in determining the susceptibility to nephropathy in type 2 diabetes patients. Export

@article{citeulike:3718885, abstract = {BACKGROUND: The IL-10 promoter polymorphisms -1082G/A, -819C/T, and -592C/A have been consistently associated with type 2 diabetes (T2DM). We examined whether these polymorphisms variants are also associated with progression of diabetic nephropathy (DN). METHODS: These promoter variants were genotyped in 917 T2DM patients comprising 515 DN patients and 402 control patients without nephropathy (DWN), together with 748 non-diabetic control subjects. Haplotype analysis and multivariate regression analysis were employed in assessing the contribution of IL-10 haplotypes to DN risk, using genotype, clinical and biochemical profile, and their interactions as predictors of DN. RESULTS: Carriers of mutant -592A and -819T alleles, and -819T/T, -592A/A, and -819C/T genotypes were more frequent in T2DM. However, the -819C/T genotype appeared to be protective of DN, since lower frequency -819T allele and -819C/T genotype were seen in DN patients. Regression analysis identified -1082G/-819T/-592A (GTA) and -1082G/-819T/-592C (GTC) haplotypes as DN-protective haplotypes. Relative to the -1082G/-819C/-592C haplotype, GTA [P = 0.044; odds ratio (OR) = 0.54, 95\% confidence interval (CI): 0.30-0.98] and GTC (P = 0.045; OR = 0.56, 95\% CI: 0.31-0.99) haplotypes were associated with decreased odds ratio (OR) for DN, after controlling for a number of covariates (age, sex, body mass index (BMI), hypertension, glucose, HbA(1c), DN duration, total cholesterol). CONCLUSIONS: Our results indicate that genetic variations at the IL-10 promoter influence the risk of nephropathy in T2DM patients and thus represent a potential DN genetic-susceptibility locus worthy of replication.}, author = {Mtiraoui, Nabil and Ezzidi, Intissar and Kacem, Maha and Ben Hadj Mohamed, Manel and Chaieb, Molka and Haj Jilani, Aoutef Bel B. and Mahjoub, Touhami and Almawi, Wassim Y.}, citeulike-article-id = {3718885}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/dmrr.892}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19031431}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19031431}, day = {17}, doi = {10.1002/dmrr.892}, issn = {1520-7560}, journal = {Diabetes/metabolism research and reviews}, keywords = {diabetic-complications, genetic-polymorphism, il-10}, month = {January}, number = {1}, pages = {57--63}, posted-at = {2009-11-04 23:43:21}, priority = {2}, title = {Predictive value of interleukin-10 promoter genotypes and haplotypes in determining the susceptibility to nephropathy in type 2 diabetes patients.}, url = {http://dx.doi.org/10.1002/dmrr.892}, volume = {25}, year = {2009} }

TY - JOUR ID - citeulike:3718885 L3 - citeulike-article-id:3718885 N2 - BACKGROUND: The IL-10 promoter polymorphisms -1082G/A, -819C/T, and -592C/A have been consistently associated with type 2 diabetes (T2DM). We examined whether these polymorphisms variants are also associated with progression of diabetic nephropathy (DN). METHODS: These promoter variants were genotyped in 917 T2DM patients comprising 515 DN patients and 402 control patients without nephropathy (DWN), together with 748 non-diabetic control subjects. Haplotype analysis and multivariate regression analysis were employed in assessing the contribution of IL-10 haplotypes to DN risk, using genotype, clinical and biochemical profile, and their interactions as predictors of DN. RESULTS: Carriers of mutant -592A and -819T alleles, and -819T/T, -592A/A, and -819C/T genotypes were more frequent in T2DM. However, the -819C/T genotype appeared to be protective of DN, since lower frequency -819T allele and -819C/T genotype were seen in DN patients. Regression analysis identified -1082G/-819T/-592A (GTA) and -1082G/-819T/-592C (GTC) haplotypes as DN-protective haplotypes. Relative to the -1082G/-819C/-592C haplotype, GTA [P = 0.044; odds ratio (OR) = 0.54, 95% confidence interval (CI): 0.30-0.98] and GTC (P = 0.045; OR = 0.56, 95% CI: 0.31-0.99) haplotypes were associated with decreased odds ratio (OR) for DN, after controlling for a number of covariates (age, sex, body mass index (BMI), hypertension, glucose, HbA(1c), DN duration, total cholesterol). CONCLUSIONS: Our results indicate that genetic variations at the IL-10 promoter influence the risk of nephropathy in T2DM patients and thus represent a potential DN genetic-susceptibility locus worthy of replication. IS - 1 JF - Diabetes/metabolism research and reviews SN - 1520-7560 EP - 63 TI - Predictive value of interleukin-10 promoter genotypes and haplotypes in determining the susceptibility to nephropathy in type 2 diabetes patients. VL - 25 SP - 57 KW - diabetic-complications KW - genetic-polymorphism KW - il-10 AU - Mtiraoui, Nabil AU - Ezzidi, Intissar AU - Kacem, Maha AU - Ben Hadj Mohamed, Manel AU - Chaieb, Molka AU - Haj Jilani, Aoutef Bel AU - Mahjoub, Touhami AU - Almawi, Wassim PY - 2009/01/17/ UR - http://dx.doi.org/10.1002/dmrr.892 ER -