Interleukin-10-592C/A, -819C/T and -1082A/G promoter variants affect the susceptibility to nephropathy in Tunisian type 2 diabetes (T2DM) patients. Export

@article{citeulike:4044497, abstract = {BACKGROUND: The Interleukin (IL)-10 polymorphic variants -1082G/A, -819C/T and -592C/A were linked with obesity, metabolic syndrome, and type 2 diabetes (T2DM). We investigated the hypothesis that IL-10 promoter polymorphisms may be associated with the progression of diabetic nephropathy (DN). DESIGN: Case-controlled study. PATIENTS: Study subjects comprised of 515 DN patients, and 402 normoalbuminuric (DWN) T2DM patients. MEASUREMENTS: IL-10 genotyping was done by PCR-based assays, and the contributions of the IL-10 polymorphic variants to DN were analysed by haplotype analysis and multivariate regression analysis. RESULTS: Decreased prevalence of (mutant) -819T allele and -819C/T genotype was seen in DN patients; neither the -1082G/A nor the -592C/A polymorphism was associated with DN. Three-loci haplotype (-1082GA/-819CT/-592CA) analysis identified GTC as DN-protective haplotype. Multivariate regression analysis confirmed the association of GTC haplotype (P = 0.045; OR = 0.56, 95\% CI: 0.31-0.99), and in addition identified GTA haplotype (P = 0.044; OR = 0.54, 95\% CI: 0.30-0.98) as independent predictors of DN after controlling for a number of covariates (age, sex, BMI; hypertension, glucose, HbA1c, DN duration, total cholesterol, medications). CONCLUSION: This study suggests that IL-10 promoter polymorphism influence the risk of nephropathy in Tunisian T2DM patients.}, author = {Ezzidi, Intissar and Mtiraoui, Nabil and Kacem, Maha and Mallat, Samir G. and Mohamed, Manel Ben Hadj B. and Chaieb, Molka and Mahjoub, Touhami and Almawi, Wassim Y.}, citeulike-article-id = {4044497}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1365-2265.2008.03337.x}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/bsc/cend/2009/00000070/00000003/art00010}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18616700}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18616700}, doi = {10.1111/j.1365-2265.2008.03337.x}, issn = {1365-2265}, journal = {Clinical endocrinology}, keywords = {diabetic-complications, genetic-polymorphism, il-10}, month = {March}, number = {3}, pages = {401--407}, posted-at = {2009-11-04 23:43:56}, priority = {2}, publisher = {Blackwell Publishing}, title = {Interleukin-10-592C/A, -819C/T and -1082A/G promoter variants affect the susceptibility to nephropathy in Tunisian type 2 diabetes (T2DM) patients.}, url = {http://dx.doi.org/10.1111/j.1365-2265.2008.03337.x}, volume = {70}, year = {2009} }

TY - JOUR ID - citeulike:4044497 L3 - citeulike-article-id:4044497 N2 - BACKGROUND: The Interleukin (IL)-10 polymorphic variants -1082G/A, -819C/T and -592C/A were linked with obesity, metabolic syndrome, and type 2 diabetes (T2DM). We investigated the hypothesis that IL-10 promoter polymorphisms may be associated with the progression of diabetic nephropathy (DN). DESIGN: Case-controlled study. PATIENTS: Study subjects comprised of 515 DN patients, and 402 normoalbuminuric (DWN) T2DM patients. MEASUREMENTS: IL-10 genotyping was done by PCR-based assays, and the contributions of the IL-10 polymorphic variants to DN were analysed by haplotype analysis and multivariate regression analysis. RESULTS: Decreased prevalence of (mutant) -819T allele and -819C/T genotype was seen in DN patients; neither the -1082G/A nor the -592C/A polymorphism was associated with DN. Three-loci haplotype (-1082GA/-819CT/-592CA) analysis identified GTC as DN-protective haplotype. Multivariate regression analysis confirmed the association of GTC haplotype (P = 0.045; OR = 0.56, 95% CI: 0.31-0.99), and in addition identified GTA haplotype (P = 0.044; OR = 0.54, 95% CI: 0.30-0.98) as independent predictors of DN after controlling for a number of covariates (age, sex, BMI; hypertension, glucose, HbA1c, DN duration, total cholesterol, medications). CONCLUSION: This study suggests that IL-10 promoter polymorphism influence the risk of nephropathy in Tunisian T2DM patients. IS - 3 JF - Clinical endocrinology SN - 1365-2265 EP - 407 TI - Interleukin-10-592C/A, -819C/T and -1082A/G promoter variants affect the susceptibility to nephropathy in Tunisian type 2 diabetes (T2DM) patients. PB - Blackwell Publishing VL - 70 SP - 401 KW - diabetic-complications KW - genetic-polymorphism KW - il-10 AU - Ezzidi, Intissar AU - Mtiraoui, Nabil AU - Kacem, Maha AU - Mallat, Samir AU - Mohamed, Manel Ben Hadj AU - Chaieb, Molka AU - Mahjoub, Touhami AU - Almawi, Wassim PY - 2009/03// UR - http://dx.doi.org/10.1111/j.1365-2265.2008.03337.x ER -