Initiation of DNA replication at CpG islands in mammalian chromosomes. Export

@article{citeulike:3292379, abstract = {CpG islands are G+C-rich regions approximately 1 kb long that are free of methylation and contain the promoters of many mammalian genes. Analysis of in vivo replication intermediates at three hamster genes and one human gene showed that the CpG island regions, but not their flanks, were present in very short nascent strands, suggesting that they are replication origins (ORIs). CpG island-like fragments were enriched in a population of short nascent strands from human erythroleukaemic cells, suggesting that islands constitute a significant fraction of endogenous ORIs. Correspondingly, bulk CpG islands were found to replicate coordinately early in S phase. Our results imply that CpG islands are initiation sites for both transcription and DNA replication, and may represent genomic footprints of replication initiation.}, address = {Instituto de Microbiolog\'{\i}a Bioqu\'{\i}mica, CSIC/Universidad de Salamanca, Edificio Departamental, Campus Miguel de Unamuno, 37007-Salamanca, Spain.}, author = {Delgado, S. and G\'{o}mez, M. and Bird, A. and Antequera, F.}, citeulike-article-id = {3292379}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9545253}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9545253}, day = {15}, issn = {0261-4189}, journal = {The EMBO journal}, keywords = {cgi, replication}, month = {April}, number = {8}, pages = {2426--2435}, posted-at = {2008-09-19 10:25:57}, priority = {2}, title = {Initiation of DNA replication at CpG islands in mammalian chromosomes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9545253}, volume = {17}, year = {1998} }

TY - JOUR ID - citeulike:3292379 L3 - citeulike-article-id:3292379 N2 - CpG islands are G+C-rich regions approximately 1 kb long that are free of methylation and contain the promoters of many mammalian genes. Analysis of in vivo replication intermediates at three hamster genes and one human gene showed that the CpG island regions, but not their flanks, were present in very short nascent strands, suggesting that they are replication origins (ORIs). CpG island-like fragments were enriched in a population of short nascent strands from human erythroleukaemic cells, suggesting that islands constitute a significant fraction of endogenous ORIs. Correspondingly, bulk CpG islands were found to replicate coordinately early in S phase. Our results imply that CpG islands are initiation sites for both transcription and DNA replication, and may represent genomic footprints of replication initiation. IS - 8 JF - The EMBO journal SN - 0261-4189 AD - Instituto de Microbiología Bioquímica, CSIC/Universidad de Salamanca, Edificio Departamental, Campus Miguel de Unamuno, 37007-Salamanca, Spain. EP - 2435 TI - Initiation of DNA replication at CpG islands in mammalian chromosomes. VL - 17 SP - 2426 KW - cgi KW - replication AU - Delgado, S AU - Gómez, M AU - Bird, A AU - Antequera, F PY - 1998/04/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9545253 ER -