Architecture of the protein-conducting channel associated with the translating 80S ribosome. Export

@article{citeulike:1933731, abstract = {In vitro assembled yeast ribosome-nascent chain complexes (RNCs) containing a signal sequence in the nascent chain were immunopurified and reconstituted with the purified protein-conducting channel (PCC) of yeast endoplasmic reticulum, the Sec61 complex. A cryo-EM reconstruction of the RNC-Sec61 complex at 15.4 A resolution shows a tRNA in the P site. Distinct rRNA elements and proteins of the large ribosomal subunit form four connections with the PCC across a gap of about 10-20 A. Binding of the PCC influences the position of the highly dynamic rRNA expansion segment 27. The RNC-bound Sec61 complex has a compact appearance and was estimated to be a trimer. We propose a binary model of cotranslational translocation entailing only two basic functional states of the translating ribosome-channel complex.}, address = {Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. roland.beckmann@charite.de}, author = {Beckmann, R. and Spahn, C. M. and Eswar, N. and Helmers, J. and Penczek, P. A. and Sali, A. and Frank, J. and Blobel, G.}, citeulike-article-id = {1933731}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11701126}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11701126}, day = {2}, issn = {0092-8674}, journal = {Cell}, keywords = {gartmann, sec61}, month = {November}, number = {3}, pages = {361--372}, posted-at = {2009-10-30 14:50:19}, priority = {2}, title = {Architecture of the protein-conducting channel associated with the translating 80S ribosome.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11701126}, volume = {107}, year = {2001} }

TY - JOUR ID - citeulike:1933731 L3 - citeulike-article-id:1933731 N2 - In vitro assembled yeast ribosome-nascent chain complexes (RNCs) containing a signal sequence in the nascent chain were immunopurified and reconstituted with the purified protein-conducting channel (PCC) of yeast endoplasmic reticulum, the Sec61 complex. A cryo-EM reconstruction of the RNC-Sec61 complex at 15.4 A resolution shows a tRNA in the P site. Distinct rRNA elements and proteins of the large ribosomal subunit form four connections with the PCC across a gap of about 10-20 A. Binding of the PCC influences the position of the highly dynamic rRNA expansion segment 27. The RNC-bound Sec61 complex has a compact appearance and was estimated to be a trimer. We propose a binary model of cotranslational translocation entailing only two basic functional states of the translating ribosome-channel complex. IS - 3 JF - Cell SN - 0092-8674 AD - Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. roland.beckmann@charite.de EP - 372 TI - Architecture of the protein-conducting channel associated with the translating 80S ribosome. VL - 107 SP - 361 KW - gartmann KW - sec61 AU - Beckmann, R AU - Spahn, CM AU - Eswar, N AU - Helmers, J AU - Penczek, PA AU - Sali, A AU - Frank, J AU - Blobel, G PY - 2001/11/02/ UR - http://view.ncbi.nlm.nih.gov/pubmed/11701126 ER -