Selective variations in vivo of VH3 and VH1 gene family expression in peripheral B cell IgM, IgD and IgG during HIV infection. Export

@article{citeulike:1621860, abstract = {We have analyzed the expression of VH gene families in IgM, IgD and IgG of peripheral blood B cells from a group of HIV-infected patients. CD19+CD20+ cells were purified and anchored reverse transcriptase-polymerase chain reaction products were hybridized with VH gene family probes. IgM, IgD and IgG that expressed a VH3 gene family segment, were decreased in patients with low CD4 counts and to a greater extend in patients with AIDS symptoms (up to 85\% for IgG) compared to adult healthy donors. This was correlated with elevated levels of IgM and IgG encoded by a VH1 gene family segment (around 60\% for IgG). These results confirm and extend previous work that has detected the VH3 gene family under-representation in HIV infection. Here, we show that, in vivo, this phenomenon actually affects the different B cell populations of the peripheral blood: IgM+ or IgG+ B cells and also IgM+IgD+ naive B cells. In the course of HIV infection, this results in their gradual depletion. Data presented here strengthen the hypothesis that a B-cell superantigen exists in HIV infection. These pronounced variations of the normally most-expressed VH gene family may be related to B cell abnormalities detected in HIV-infected patients.}, address = {D\'{e}partement d'Immunologie, Institut Pasteur, Paris, France.}, author = {David, D. and Demaison, C. and Bani, L. and Zouali, M. and Th\`{e}ze, J.}, citeulike-article-id = {1621860}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7614977}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7614977}, issn = {0014-2980}, journal = {Eur J Immunol}, keywords = {gene, hiv, usage}, month = {June}, number = {6}, pages = {1524--1528}, posted-at = {2007-09-05 07:12:22}, priority = {2}, title = {Selective variations in vivo of VH3 and VH1 gene family expression in peripheral B cell IgM, IgD and IgG during HIV infection.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7614977}, volume = {25}, year = {1995} }

TY - JOUR ID - citeulike:1621860 L3 - citeulike-article-id:1621860 N2 - We have analyzed the expression of VH gene families in IgM, IgD and IgG of peripheral blood B cells from a group of HIV-infected patients. CD19+CD20+ cells were purified and anchored reverse transcriptase-polymerase chain reaction products were hybridized with VH gene family probes. IgM, IgD and IgG that expressed a VH3 gene family segment, were decreased in patients with low CD4 counts and to a greater extend in patients with AIDS symptoms (up to 85% for IgG) compared to adult healthy donors. This was correlated with elevated levels of IgM and IgG encoded by a VH1 gene family segment (around 60% for IgG). These results confirm and extend previous work that has detected the VH3 gene family under-representation in HIV infection. Here, we show that, in vivo, this phenomenon actually affects the different B cell populations of the peripheral blood: IgM+ or IgG+ B cells and also IgM+IgD+ naive B cells. In the course of HIV infection, this results in their gradual depletion. Data presented here strengthen the hypothesis that a B-cell superantigen exists in HIV infection. These pronounced variations of the normally most-expressed VH gene family may be related to B cell abnormalities detected in HIV-infected patients. IS - 6 JF - Eur J Immunol SN - 0014-2980 AD - Département d'Immunologie, Institut Pasteur, Paris, France. EP - 1528 TI - Selective variations in vivo of VH3 and VH1 gene family expression in peripheral B cell IgM, IgD and IgG during HIV infection. VL - 25 SP - 1524 KW - gene KW - hiv KW - usage AU - David, D AU - Demaison, C AU - Bani, L AU - Zouali, M AU - Thèze, J PY - 1995/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/7614977 ER -