Analysis of Ig VH region genes encoding IgE antibodies in splenic B lymphocytes of a patient with asthma. Export

@article{citeulike:2325331, abstract = {An atopic patient with hypersensitivity against house dust mite died as a result of an asthmatic attack. A portion of the spleen was obtained and was used to analyze the spectrum of Ig heavy chain V regions involved in encoding IgE Abs. A nested PCR technique generated 14 cloned VH sequences that had distinct CDR3 regions; 5 of 14 were derived from the minor VH5 family, and the remainder derived from the larger families, VH3 (6 of 14) and VH4 (3 of 14). One of the VH3-derived sequences was present as a repeated sequence in three clones. A control PCR with the same VH primers in combination with JH primers yielded only 1 of 13 sequences from VH5, indicating preferential VH5 usage only for IgE. Analysis of VH5-C epsilon sequences revealed usage of a single gene, DP73, with extensive mutations and several "hot spots" containing common replacement amino acids. However, there was no concentration of replacement mutations in the CDRs, which conventionally would indicate a role for Ag selection. The VH3 and VH4 genes in combination with C epsilon also harbored extensive somatic mutations. From these findings in splenic B lymphocytes, and those of a previous study of blood lymphocytes, it seems that preferential usage of VH5 genes and extensive somatic hypermutation are characteristic of B cells synthesizing IgE in patients with allergic disease.}, address = {Molecular Immunology Group, Tenovus Laboratory, Southampton University Hospitals, United Kingdom.}, author = {Snow, R. E. and Chapman, C. J. and Frew, A. J. and Holgate, S. T. and Stevenson, F. K.}, citeulike-article-id = {2325331}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7730656}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7730656}, day = {15}, issn = {0022-1767}, journal = {J Immunol}, keywords = {asthma, usage, vgene}, month = {May}, number = {10}, pages = {5576--5581}, posted-at = {2008-02-03 10:25:31}, priority = {2}, title = {Analysis of Ig VH region genes encoding IgE antibodies in splenic B lymphocytes of a patient with asthma.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7730656}, volume = {154}, year = {1995} }

TY - JOUR ID - citeulike:2325331 L3 - citeulike-article-id:2325331 N2 - An atopic patient with hypersensitivity against house dust mite died as a result of an asthmatic attack. A portion of the spleen was obtained and was used to analyze the spectrum of Ig heavy chain V regions involved in encoding IgE Abs. A nested PCR technique generated 14 cloned VH sequences that had distinct CDR3 regions; 5 of 14 were derived from the minor VH5 family, and the remainder derived from the larger families, VH3 (6 of 14) and VH4 (3 of 14). One of the VH3-derived sequences was present as a repeated sequence in three clones. A control PCR with the same VH primers in combination with JH primers yielded only 1 of 13 sequences from VH5, indicating preferential VH5 usage only for IgE. Analysis of VH5-C epsilon sequences revealed usage of a single gene, DP73, with extensive mutations and several "hot spots" containing common replacement amino acids. However, there was no concentration of replacement mutations in the CDRs, which conventionally would indicate a role for Ag selection. The VH3 and VH4 genes in combination with C epsilon also harbored extensive somatic mutations. From these findings in splenic B lymphocytes, and those of a previous study of blood lymphocytes, it seems that preferential usage of VH5 genes and extensive somatic hypermutation are characteristic of B cells synthesizing IgE in patients with allergic disease. IS - 10 JF - J Immunol SN - 0022-1767 AD - Molecular Immunology Group, Tenovus Laboratory, Southampton University Hospitals, United Kingdom. EP - 5581 TI - Analysis of Ig VH region genes encoding IgE antibodies in splenic B lymphocytes of a patient with asthma. VL - 154 SP - 5576 KW - asthma KW - usage KW - vgene AU - Snow, RE AU - Chapman, CJ AU - Frew, AJ AU - Holgate, ST AU - Stevenson, FK PY - 1995/05/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/7730656 ER -