Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing. Export

@article{citeulike:839672, abstract = {The humoral immune system in higher vertebrates is unique in its ability to generate highly diverse antibody responses against most pathogens as well as against certain malignancies. Several technologies have been developed to exploit this vast source of potentially therapeutic antibodies, including hybridoma technology, phage display and yeast display. Here, we present a novel, high-throughput technology (the Symplex Technology) for rapid direct cloning and identification of human antigen-specific high-affinity antibodies from single antibody-producing cells of immune individuals. The utility of the technology was demonstrated by isolation of diverse sets of unique high-affinity antibodies against tetanus toxoid and influenza virus from immunized volunteers. Hence, the Symplex Technology is a new method for the rapid isolation of high-affinity antibodies directly from humans.}, address = {Symphogen A/S, Elektrovej, Building 375, DK-2800 Lyngby, Denmark.}, author = {Meijer, P. J. and Andersen, P. S. and Haahr Hansen, M. and Steinaa, L. and Jensen, A. and Lantto, J. and Oleksiewicz, M. B. and Tengbjerg, K. and Poulsen, T. R. and Coljee, V. W. and Bregenholt, S. and Haurum, J. S. and Nielsen, L. S.}, citeulike-article-id = {839672}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jmb.2006.02.040}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16563430}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16563430}, day = {5}, doi = {10.1016/j.jmb.2006.02.040}, issn = {0022-2836}, journal = {J Mol Biol}, keywords = {high, repertoire, through-put}, month = {May}, number = {3}, pages = {764--772}, posted-at = {2006-09-12 00:15:53}, priority = {2}, title = {Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing.}, url = {http://dx.doi.org/10.1016/j.jmb.2006.02.040}, volume = {358}, year = {2006} }

TY - JOUR ID - citeulike:839672 L3 - citeulike-article-id:839672 N2 - The humoral immune system in higher vertebrates is unique in its ability to generate highly diverse antibody responses against most pathogens as well as against certain malignancies. Several technologies have been developed to exploit this vast source of potentially therapeutic antibodies, including hybridoma technology, phage display and yeast display. Here, we present a novel, high-throughput technology (the Symplex Technology) for rapid direct cloning and identification of human antigen-specific high-affinity antibodies from single antibody-producing cells of immune individuals. The utility of the technology was demonstrated by isolation of diverse sets of unique high-affinity antibodies against tetanus toxoid and influenza virus from immunized volunteers. Hence, the Symplex Technology is a new method for the rapid isolation of high-affinity antibodies directly from humans. IS - 3 JF - J Mol Biol SN - 0022-2836 AD - Symphogen A/S, Elektrovej, Building 375, DK-2800 Lyngby, Denmark. EP - 772 TI - Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing. VL - 358 SP - 764 KW - high KW - repertoire KW - through-put AU - Meijer, PJ AU - Andersen, PS AU - Haahr Hansen, M AU - Steinaa, L AU - Jensen, A AU - Lantto, J AU - Oleksiewicz, MB AU - Tengbjerg, K AU - Poulsen, TR AU - Coljee, VW AU - Bregenholt, S AU - Haurum, JS AU - Nielsen, LS PY - 2006/05/05/ UR - http://dx.doi.org/10.1016/j.jmb.2006.02.040 ER -