Microdialysis separately monitors myocardial interstitial myoglobin during ischemia and reperfusion. Export

@article{citeulike:379599, abstract = {Direct monitoring of myoglobin efflux during ischemia and reperfusion has been limited because of inherent sample collection problems in the ischemic region. Recently, the cardiac dialysis technique has offered a powerful method for monitoring myocardial interstitial levels of low-molecular-weight compounds in the cardiac ischemic region. In the present study, we extended the molecular target to high-molecular-weight compounds by use of microdialysis probes with a high-molecular-mass cutoff and monitored myocardial interstitial myoglobin levels. A dialysis probe was implanted in the left ventricular free wall in anesthetized rabbits. The main coronary artery was occluded for 60 or 120 min. We examined the effects of myocardial ischemia and reperfusion on myocardial interstitial myoglobin levels. Interstitial myoglobin increased within 15 min of ischemia and continued to increase during 120 min of ischemia, whereas blood myoglobin increased at 45 min of ischemia. Lactate and myoglobin in the interstitial space increased during the same period. At 60 min of ischemia, reperfusion markedly accelerated interstitial myoglobin release. The interstitial myoglobin level was fivefold higher at 0-15 min of reperfusion than at 60-75 min of coronary occlusion. The dialysis technique permits earlier detection of myoglobin release and separately monitors myoglobin release during ischemia and reperfusion. Myocardial interstitial myoglobin levels can serve as an index of myocardial injury evoked by ischemia or reperfusion.}, address = {Department Anesthesiology, Shiga University of Medical Science, Otsu, Japan.}, author = {Kitagawa, H. and Yamazaki, T. and Akiyama, T. and Sugimachi, M. and Sunagawa, K. and Mori, H.}, citeulike-article-id = {379599}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajpheart.01207.2004}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15833797}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15833797}, doi = {10.1152/ajpheart.01207.2004}, issn = {0363-6135}, journal = {Am J Physiol Heart Circ Physiol}, keywords = {efflux, ischemia, myocardial, myoglobin, reperfusion}, month = {August}, number = {2}, posted-at = {2005-11-03 18:04:14}, priority = {2}, title = {Microdialysis separately monitors myocardial interstitial myoglobin during ischemia and reperfusion.}, url = {http://dx.doi.org/10.1152/ajpheart.01207.2004}, volume = {289}, year = {2005} }

TY - JOUR ID - citeulike:379599 L3 - citeulike-article-id:379599 N2 - Direct monitoring of myoglobin efflux during ischemia and reperfusion has been limited because of inherent sample collection problems in the ischemic region. Recently, the cardiac dialysis technique has offered a powerful method for monitoring myocardial interstitial levels of low-molecular-weight compounds in the cardiac ischemic region. In the present study, we extended the molecular target to high-molecular-weight compounds by use of microdialysis probes with a high-molecular-mass cutoff and monitored myocardial interstitial myoglobin levels. A dialysis probe was implanted in the left ventricular free wall in anesthetized rabbits. The main coronary artery was occluded for 60 or 120 min. We examined the effects of myocardial ischemia and reperfusion on myocardial interstitial myoglobin levels. Interstitial myoglobin increased within 15 min of ischemia and continued to increase during 120 min of ischemia, whereas blood myoglobin increased at 45 min of ischemia. Lactate and myoglobin in the interstitial space increased during the same period. At 60 min of ischemia, reperfusion markedly accelerated interstitial myoglobin release. The interstitial myoglobin level was fivefold higher at 0-15 min of reperfusion than at 60-75 min of coronary occlusion. The dialysis technique permits earlier detection of myoglobin release and separately monitors myoglobin release during ischemia and reperfusion. Myocardial interstitial myoglobin levels can serve as an index of myocardial injury evoked by ischemia or reperfusion. IS - 2 JF - Am J Physiol Heart Circ Physiol SN - 0363-6135 AD - Department Anesthesiology, Shiga University of Medical Science, Otsu, Japan. TI - Microdialysis separately monitors myocardial interstitial myoglobin during ischemia and reperfusion. VL - 289 KW - efflux KW - ischemia KW - myocardial KW - myoglobin KW - reperfusion AU - Kitagawa, H AU - Yamazaki, T AU - Akiyama, T AU - Sugimachi, M AU - Sunagawa, K AU - Mori, H PY - 2005/08// UR - http://dx.doi.org/10.1152/ajpheart.01207.2004 ER -