Morphological, electrophysiological, and synaptic properties of corticocallosal pyramidal cells in the neonatal rat neocortex. Export

@article{citeulike:3400927, abstract = {Neocortical pyramidal cells (PCs) project to various cortical and subcortical targets. In layer V, the population of thick tufted PCs (TTCs) projects to subcortical targets such as the tectum, brainstem, and spinal cord. Another population of layer V PCs projects via the corpus callosum to the contralateral neocortical hemisphere mediating information transfer between the hemispheres. This subpopulation (corticocallosally projecting cells [CCPs]) has been previously described in terms of their morphological properties, but less is known about their electrophysiological properties, and their synaptic connectivity is unknown. We studied the morphological, electrophysiological, and synaptic properties of CCPs by retrograde labeling with fluorescent microbeads in P13-P16 Wistar rats. CCPs were characterized by shorter, untufted apical dendrites, which reached only up to layers II/III, confirming previous reports. Synaptic connections between CCPs were different from those observed between TTCs, both in probability of occurrence and dynamic properties. We found that the CCP network is about 4 times less interconnected than the TTC network and the probability of release is 24\% smaller, resulting in a more linear synaptic transmission. The study shows that layer V pyramidal neurons projecting to different targets form subnetworks with specialized connectivity profiles, in addition to the specialized morphological and electrophysiological intrinsic properties.}, author = {Le B\'{e}, Jean-Vincent V. and Silberberg, Gilad and Wang, Yun and Markram, Henry}, citeulike-article-id = {3400927}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/cercor/bhl127}, citeulike-linkout-1 = {http://cercor.oxfordjournals.org/content/17/9/2204.abstract}, citeulike-linkout-2 = {http://cercor.oxfordjournals.org/content/17/9/2204.full.pdf}, citeulike-linkout-3 = {http://cercor.oxfordjournals.org/cgi/content/abstract/17/9/2204}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/17124287}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=17124287}, day = {1}, doi = {10.1093/cercor/bhl127}, issn = {1047-3211}, journal = {Cerebral cortex (New York, N.Y. : 1991)}, keywords = {cortex, ephys, projection, rat}, month = {September}, number = {9}, pages = {2204--2213}, posted-at = {2009-09-25 03:47:28}, priority = {2}, title = {Morphological, electrophysiological, and synaptic properties of corticocallosal pyramidal cells in the neonatal rat neocortex.}, url = {http://dx.doi.org/10.1093/cercor/bhl127}, volume = {17}, year = {2007} }

TY - JOUR ID - citeulike:3400927 L3 - citeulike-article-id:3400927 N2 - Neocortical pyramidal cells (PCs) project to various cortical and subcortical targets. In layer V, the population of thick tufted PCs (TTCs) projects to subcortical targets such as the tectum, brainstem, and spinal cord. Another population of layer V PCs projects via the corpus callosum to the contralateral neocortical hemisphere mediating information transfer between the hemispheres. This subpopulation (corticocallosally projecting cells [CCPs]) has been previously described in terms of their morphological properties, but less is known about their electrophysiological properties, and their synaptic connectivity is unknown. We studied the morphological, electrophysiological, and synaptic properties of CCPs by retrograde labeling with fluorescent microbeads in P13-P16 Wistar rats. CCPs were characterized by shorter, untufted apical dendrites, which reached only up to layers II/III, confirming previous reports. Synaptic connections between CCPs were different from those observed between TTCs, both in probability of occurrence and dynamic properties. We found that the CCP network is about 4 times less interconnected than the TTC network and the probability of release is 24% smaller, resulting in a more linear synaptic transmission. The study shows that layer V pyramidal neurons projecting to different targets form subnetworks with specialized connectivity profiles, in addition to the specialized morphological and electrophysiological intrinsic properties. IS - 9 JF - Cerebral cortex (New York, N.Y. : 1991) SN - 1047-3211 EP - 2213 TI - Morphological, electrophysiological, and synaptic properties of corticocallosal pyramidal cells in the neonatal rat neocortex. VL - 17 SP - 2204 KW - cortex KW - ephys KW - projection KW - rat AU - Le Bé, Jean-Vincent AU - Silberberg, Gilad AU - Wang, Yun AU - Markram, Henry PY - 2007/09/01/ UR - http://dx.doi.org/10.1093/cercor/bhl127 ER -