Basic Principles and Technologies for Deciphering the Genetic Map of Cancer Export

@article{citeulike:3841091, abstract = {Abstract\ \ The progress achieved in the field of genomics in recent years is leading medicine to adopt a personalized model in which the knowledge of individual DNA alterations will allow a targeted approach to cancer. Using pancreatic cancer as a model, we discuss herein the fundamentals that need to be considered for the high throughput and global identification of mutations. These include patient-related issues, sample collection, DNA isolation, gene selection, primer design, and sequencing techniques. We also describe the possible applications of the discovery of DNA changes to the approach of this disease and cite preliminary efforts where the knowledge has been translated into the clinical or preclinical setting.}, author = {Voidonikolas, Georgios and Kreml, Stephanie and Chen, Changyi and Fisher, William and Charles Brunicardi, F. and Gibbs, Richard and Gingras, Marie-Claude}, citeulike-article-id = {3841091}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00268-008-9851-y}, citeulike-linkout-1 = {http://www.springerlink.com/content/l840253530302866}, day = {1}, doi = {10.1007/s00268-008-9851-y}, journal = {World Journal of Surgery}, keywords = {cancer, list, management, p4medicine}, month = {April}, number = {4}, pages = {615--629}, posted-at = {2009-09-14 23:00:54}, priority = {2}, title = {Basic Principles and Technologies for Deciphering the Genetic Map of Cancer}, url = {http://dx.doi.org/10.1007/s00268-008-9851-y}, volume = {33}, year = {2009} }

TY - JOUR ID - citeulike:3841091 L3 - citeulike-article-id:3841091 N2 - Abstract  The progress achieved in the field of genomics in recent years is leading medicine to adopt a personalized model in which the knowledge of individual DNA alterations will allow a targeted approach to cancer. Using pancreatic cancer as a model, we discuss herein the fundamentals that need to be considered for the high throughput and global identification of mutations. These include patient-related issues, sample collection, DNA isolation, gene selection, primer design, and sequencing techniques. We also describe the possible applications of the discovery of DNA changes to the approach of this disease and cite preliminary efforts where the knowledge has been translated into the clinical or preclinical setting. IS - 4 JF - World Journal of Surgery EP - 629 TI - Basic Principles and Technologies for Deciphering the Genetic Map of Cancer VL - 33 SP - 615 KW - cancer KW - list KW - management KW - p4medicine AU - Voidonikolas, Georgios AU - Kreml, Stephanie AU - Chen, Changyi AU - Fisher, William AU - Charles Brunicardi, F AU - Gibbs, Richard AU - Gingras, Marie-Claude PY - 2009/04/01/ UR - http://dx.doi.org/10.1007/s00268-008-9851-y ER -