Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by the MLL Fusion Partner TET1 Export

@article{citeulike:4368811, abstract = {DNA cytosine methylation is crucial for retrotransposon silencing and mammalian development. In a computational search for enzymes that could modify 5-methylcytosine (5mC), we identified TET proteins as mammalian homologs of the trypanosome proteins JBP1 and JBP2, which have been proposed to oxidize the 5-methyl group of thymine. We show here that TET1, a fusion partner of the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that catalyzes conversion of 5mC to 5-hydroxymethylcytosine (hmC) in cultured cells and in vitro. hmC is present in the genome of mouse ES cells, and hmC levels decrease upon RNAi-mediated depletion of TET1. Thus, TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC. 10.1126/science.1170116}, author = {Tahiliani, Mamta and Koh, Kian P. and Shen, Yinghua and Pastor, William A. and Bandukwala, Hozefa and Brudno, Yevgeny and Agarwal, Suneet and Iyer, Lakshminarayan M. and Liu, David R. and Aravind, L. and Rao, Anjana}, citeulike-article-id = {4368811}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1170116}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/324/5929/930}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19372391}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19372391}, day = {16}, doi = {10.1126/science.1170116}, journal = {Science}, keywords = {5, biology, cool, modification, prime}, month = {April}, pages = {1170116+}, posted-at = {2009-06-08 05:38:25}, priority = {3}, title = {Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by the MLL Fusion Partner TET1}, url = {http://dx.doi.org/10.1126/science.1170116}, year = {2009} }

TY - JOUR ID - citeulike:4368811 L3 - citeulike-article-id:4368811 N2 - DNA cytosine methylation is crucial for retrotransposon silencing and mammalian development. In a computational search for enzymes that could modify 5-methylcytosine (5mC), we identified TET proteins as mammalian homologs of the trypanosome proteins JBP1 and JBP2, which have been proposed to oxidize the 5-methyl group of thymine. We show here that TET1, a fusion partner of the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that catalyzes conversion of 5mC to 5-hydroxymethylcytosine (hmC) in cultured cells and in vitro. hmC is present in the genome of mouse ES cells, and hmC levels decrease upon RNAi-mediated depletion of TET1. Thus, TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC. 10.1126/science.1170116 JF - Science TI - Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by the MLL Fusion Partner TET1 SP - 1170116 KW - 5 KW - biology KW - cool KW - modification KW - prime AU - Tahiliani, Mamta AU - Koh, Kian AU - Shen, Yinghua AU - Pastor, William AU - Bandukwala, Hozefa AU - Brudno, Yevgeny AU - Agarwal, Suneet AU - Iyer, Lakshminarayan AU - Liu, David AU - Aravind, L AU - Rao, Anjana PY - 2009/04/16/ UR - http://dx.doi.org/10.1126/science.1170116 ER -