Differential Regulation of Dynein and Kinesin Motor Proteins by Tau Export

@article{citeulike:2260223, abstract = {Dynein and kinesin motor proteins transport cellular cargos toward opposite ends of microtubule tracks. In neurons, microtubules are abundantly decorated with microtubule-associated proteins (MAPs) such as tau. Motor proteins thus encounter MAPs frequently along their path. To determine the effects of tau on dynein and kinesin motility, we conducted single molecule studies of motor proteins moving along tau-decorated microtubules. Dynein tended to reverse direction whereas kinesin tended to detach at patches of bound tau. Kinesin was inhibited at \~{}10-fold lower tau concentration than dynein and the microtubule-binding domain of tau was sufficient to inhibit motor activity. The differential modulation of dynein and kinesin motility suggests that MAPs can spatially regulate the balance of microtubule-dependent axonal transport. 10.1126/science.1152993}, author = {Dixit, Ram and Ross, Jennifer L. and Goldman, Yale E. and Holzbaur, Erika L.}, citeulike-article-id = {2260223}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1152993}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/319/5866/1086}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18202255}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18202255}, day = {17}, doi = {10.1126/science.1152993}, journal = {Science}, keywords = {clip2, dynein, experiment, kinesin, motor, regulation}, month = {January}, pages = {1152993+}, posted-at = {2008-02-22 10:28:39}, priority = {2}, title = {Differential Regulation of Dynein and Kinesin Motor Proteins by Tau}, url = {http://dx.doi.org/10.1126/science.1152993}, year = {2008} }

TY - JOUR ID - citeulike:2260223 L3 - citeulike-article-id:2260223 N2 - Dynein and kinesin motor proteins transport cellular cargos toward opposite ends of microtubule tracks. In neurons, microtubules are abundantly decorated with microtubule-associated proteins (MAPs) such as tau. Motor proteins thus encounter MAPs frequently along their path. To determine the effects of tau on dynein and kinesin motility, we conducted single molecule studies of motor proteins moving along tau-decorated microtubules. Dynein tended to reverse direction whereas kinesin tended to detach at patches of bound tau. Kinesin was inhibited at ~10-fold lower tau concentration than dynein and the microtubule-binding domain of tau was sufficient to inhibit motor activity. The differential modulation of dynein and kinesin motility suggests that MAPs can spatially regulate the balance of microtubule-dependent axonal transport. 10.1126/science.1152993 JF - Science TI - Differential Regulation of Dynein and Kinesin Motor Proteins by Tau SP - 1152993 KW - clip2 KW - dynein KW - experiment KW - kinesin KW - motor KW - regulation AU - Dixit, Ram AU - Ross, Jennifer AU - Goldman, Yale AU - Holzbaur, Erika PY - 2008/01/17/ UR - http://dx.doi.org/10.1126/science.1152993 ER -