Single-Molecule Cut-and-Paste Surface Assembly Export

@article{citeulike:2320268, abstract = {We introduce a method for the bottom-up assembly of biomolecular structures that combines the precision of the atomic force microscope (AFM) with the selectivity of DNA hybridization. Functional units coupled to DNA oligomers were picked up from a depot area by means of a complementary DNA strand bound to an AFM tip. These units were transferred to and deposited on a target area to create basic geometrical structures, assembled from units with different functions. Each of these cut-and-paste events was characterized by single-molecule force spectroscopy and single-molecule fluorescence microscopy. Transport and deposition of more than 5000 units were achieved, with less than 10\% loss in transfer efficiency. 10.1126/science.1151424}, author = {Kufer, S. K. and Puchner, E. M. and Gumpp, H. and Liedl, T. and Gaub, H. E.}, citeulike-article-id = {2320268}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1151424}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/319/5863/594}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18239119}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18239119}, day = {1}, doi = {10.1126/science.1151424}, journal = {Science}, keywords = {afm, dna, hybridization, single\_molecule}, month = {February}, number = {5863}, pages = {594--596}, posted-at = {2008-02-12 10:04:45}, priority = {2}, title = {Single-Molecule Cut-and-Paste Surface Assembly}, url = {http://dx.doi.org/10.1126/science.1151424}, volume = {319}, year = {2008} }

TY - JOUR ID - citeulike:2320268 L3 - citeulike-article-id:2320268 N2 - We introduce a method for the bottom-up assembly of biomolecular structures that combines the precision of the atomic force microscope (AFM) with the selectivity of DNA hybridization. Functional units coupled to DNA oligomers were picked up from a depot area by means of a complementary DNA strand bound to an AFM tip. These units were transferred to and deposited on a target area to create basic geometrical structures, assembled from units with different functions. Each of these cut-and-paste events was characterized by single-molecule force spectroscopy and single-molecule fluorescence microscopy. Transport and deposition of more than 5000 units were achieved, with less than 10% loss in transfer efficiency. 10.1126/science.1151424 IS - 5863 JF - Science EP - 596 TI - Single-Molecule Cut-and-Paste Surface Assembly VL - 319 SP - 594 KW - afm KW - dna KW - hybridization KW - single_molecule AU - Kufer, SK AU - Puchner, EM AU - Gumpp, H AU - Liedl, T AU - Gaub, HE PY - 2008/02/01/ UR - http://dx.doi.org/10.1126/science.1151424 ER -