Myosin V and Kinesin act as tethers to enhance each others' processivity Export

@article{citeulike:2622611, abstract = {Organelle transport to the periphery of the cell involves coordinated transport between the processive motors kinesin and myosin V. Long-range transport takes place on microtubule tracks, whereas final delivery involves shorter actin-based movements. The concept that motors only function on their appropriate track required further investigation with the recent observation that myosin V undergoes a diffusional search on microtubules. Here we show, using single-molecule techniques, that a functional consequence of myosin V's diffusion on microtubules is a significant enhancement of the processive run length of kinesin when both motors are present on the same cargo. The degree of run length enhancement correlated with the net positive charge in loop 2 of myosin V. On actin, myosin V also undergoes longer processive runs when kinesin is present on the same cargo. The process that causes run length enhancement on both cytoskeletal tracks is electrostatic. We propose that one motor acts as a tether for the other and prevents its diffusion away from the track, thus allowing more steps to be taken before dissociation. The resulting run length enhancement likely contributes to the successful delivery of cargo in the cell. 10.1073/pnas.0711531105}, author = {Ali, Yusuf M. and Lu, Hailong and Bookwalter, Carol S. and Warshaw, David M. and Trybus, Kathleen M.}, citeulike-article-id = {2622611}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0711531105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/12/4691.full.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/12/4691.full.full.pdf}, citeulike-linkout-3 = {http://www.pnas.org/cgi/content/abstract/105/12/4691}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/18347333}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=18347333}, day = {25}, doi = {10.1073/pnas.0711531105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {experiment, kinesin, myosin, processivity}, month = {March}, number = {12}, pages = {4691--4696}, posted-at = {2008-04-02 09:24:02}, priority = {2}, title = {Myosin V and Kinesin act as tethers to enhance each others' processivity}, url = {http://dx.doi.org/10.1073/pnas.0711531105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:2622611 L3 - citeulike-article-id:2622611 N2 - Organelle transport to the periphery of the cell involves coordinated transport between the processive motors kinesin and myosin V. Long-range transport takes place on microtubule tracks, whereas final delivery involves shorter actin-based movements. The concept that motors only function on their appropriate track required further investigation with the recent observation that myosin V undergoes a diffusional search on microtubules. Here we show, using single-molecule techniques, that a functional consequence of myosin V's diffusion on microtubules is a significant enhancement of the processive run length of kinesin when both motors are present on the same cargo. The degree of run length enhancement correlated with the net positive charge in loop 2 of myosin V. On actin, myosin V also undergoes longer processive runs when kinesin is present on the same cargo. The process that causes run length enhancement on both cytoskeletal tracks is electrostatic. We propose that one motor acts as a tether for the other and prevents its diffusion away from the track, thus allowing more steps to be taken before dissociation. The resulting run length enhancement likely contributes to the successful delivery of cargo in the cell. 10.1073/pnas.0711531105 IS - 12 JF - Proceedings of the National Academy of Sciences EP - 4696 TI - Myosin V and Kinesin act as tethers to enhance each others' processivity VL - 105 SP - 4691 KW - experiment KW - kinesin KW - myosin KW - processivity AU - Ali, Yusuf AU - Lu, Hailong AU - Bookwalter, Carol AU - Warshaw, David AU - Trybus, Kathleen PY - 2008/03/25/ UR - http://dx.doi.org/10.1073/pnas.0711531105 ER -