Pulsed contractions of an actinmyosin network drive apical constriction Export

@article{citeulike:3681669, abstract = {Apical constriction facilitates epithelial sheet bending and invagination during morphogenesis. Apical constriction is conventionally thought to be driven by the continuous purse-string-like contraction of a circumferential actin and non-muscle myosin-II (myosin) belt underlying adherens junctions. However, it is unclear whether other force-generating mechanisms can drive this process. Here we show, with the use of real-time imaging and quantitative image analysis of Drosophila gastrulation, that the apical constriction of ventral furrow cells is pulsed. Repeated constrictions, which are asynchronous between neighbouring cells, are interrupted by pauses in which the constricted state of the cell apex is maintained. In contrast to the purse-string model, constriction pulses are powered by actin\&\#19;myosin network contractions that occur at the medial apical cortex and pull discrete adherens junction sites inwards. The transcription factors Twist and Snail differentially regulate pulsed constriction. Expression of snail initiates actin\&\#19;myosin network contractions, whereas expression of twist stabilizes the constricted state of the cell apex. Our results suggest a new model for apical constriction in which a cortical actin\&\#19;myosin cytoskeleton functions as a developmentally controlled subcellular ratchet to reduce apical area incrementally.}, author = {Martin, Adam C. and Kaschube, Matthias and Wieschaus, Eric F.}, citeulike-article-id = {3681669}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature07522}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature07522}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19029882}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19029882}, day = {23}, doi = {10.1038/nature07522}, issn = {0028-0836}, journal = {Nature}, keywords = {cell, clip2, experiment}, month = {November}, posted-at = {2009-02-02 08:39:26}, priority = {2}, publisher = {Nature Publishing Group}, title = {Pulsed contractions of an actinmyosin network drive apical constriction}, url = {http://dx.doi.org/10.1038/nature07522}, year = {2008} }

TY - JOUR ID - citeulike:3681669 L3 - citeulike-article-id:3681669 N2 - Apical constriction facilitates epithelial sheet bending and invagination during morphogenesis. Apical constriction is conventionally thought to be driven by the continuous purse-string-like contraction of a circumferential actin and non-muscle myosin-II (myosin) belt underlying adherens junctions. However, it is unclear whether other force-generating mechanisms can drive this process. Here we show, with the use of real-time imaging and quantitative image analysis of Drosophila gastrulation, that the apical constriction of ventral furrow cells is pulsed. Repeated constrictions, which are asynchronous between neighbouring cells, are interrupted by pauses in which the constricted state of the cell apex is maintained. In contrast to the purse-string model, constriction pulses are powered by actinmyosin network contractions that occur at the medial apical cortex and pull discrete adherens junction sites inwards. The transcription factors Twist and Snail differentially regulate pulsed constriction. Expression of snail initiates actinmyosin network contractions, whereas expression of twist stabilizes the constricted state of the cell apex. Our results suggest a new model for apical constriction in which a cortical actinmyosin cytoskeleton functions as a developmentally controlled subcellular ratchet to reduce apical area incrementally. JF - Nature SN - 0028-0836 TI - Pulsed contractions of an actinmyosin network drive apical constriction PB - Nature Publishing Group KW - cell KW - clip2 KW - experiment AU - Martin, Adam AU - Kaschube, Matthias AU - Wieschaus, Eric PY - 2008/11/23/ UR - http://dx.doi.org/10.1038/nature07522 ER -