Age-related DNA methylation changes in normal human prostate tissues. Export

@article{citeulike:1789951, abstract = {PURPOSE: Prostate cancer is a leading cause of cancer death among the aging male population but the mechanism underlying this association is unclear. Aberrant methylation of promoter CpG islands is associated with silencing of genes and age-dependent methylation of several genes has been proposed as a risk factor for sporadic cancer. We examined the extent of gene methylation in pathologically normal human prostate as a function of age. EXPERIMENTAL DESIGN: We used pyrosequencing to quantitatively analyze the methylation status of nine CpG islands in normal prostate tissue DNA from 45 organ donors and 45 patients who had undergone cystoprostatectomy for bladder cancer. We also analyzed 12 pairs of matched benign and prostate cancer tissue DNA from patients with prostate cancer. RESULTS: Linear regression analysis revealed a significant increase in promoter methylation levels correlating with age for CpG islands at RARbeta2 (r = 0.4; P < 0.0001), RASSF1A (r = 0.27; P = 0.01), GSTP1 (r = 0.59; P < 0.0001), NKX2-5 (r = 0.27; P = 0.008), and ESR1 (r = 0.244; P = 0.023) in the normal prostate tissue samples studied. A calculated average methylation (z score) at all nine CpG loci analyzed in the normal prostate tissues showed a strong correlation with age (r = 0.6; P < 0.001). Comparison of the methylation level for the matched benign and prostate cancer tissues from individual patients with prostate cancer showed significantly higher methylation in the prostate cancer tissue samples for RARbeta2 (P < 0.001), RASSF1A (P = 0.005), GSTP1 (P < 0.001), NKX2-5 (P = 0.003), ESR1 (P = 0.016), and CLSTN1 (P = 0.01). CONCLUSIONS: Our findings show aberrant hypermethylation as a function of age in the normal prostate tissues. Such age-related methylation may precede and predispose to full-blown malignancy.}, author = {Kwabi-Addo, B. and Chung, W. and Shen, L. and Ittmann, M. and Wheeler, T. and Jelinek, J. and Issa, J. P.}, citeulike-article-id = {1789951}, citeulike-linkout-0 = {http://dx.doi.org/10.1158/1078-0432.CCR-07-0085}, citeulike-linkout-1 = {http://clincancerres.aacrjournals.org/cgi/content/abstract/13/13/3796}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17606710}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17606710}, doi = {10.1158/1078-0432.CCR-07-0085}, issn = {1078-0432}, journal = {Clinical cancer research : an official journal of the American Association for Cancer Research}, month = {July}, number = {13}, pages = {3796--3802}, posted-at = {2009-06-30 09:09:16}, priority = {2}, title = {Age-related DNA methylation changes in normal human prostate tissues.}, url = {http://dx.doi.org/10.1158/1078-0432.CCR-07-0085}, volume = {13}, year = {2007} }

TY - JOUR ID - citeulike:1789951 L3 - citeulike-article-id:1789951 N2 - PURPOSE: Prostate cancer is a leading cause of cancer death among the aging male population but the mechanism underlying this association is unclear. Aberrant methylation of promoter CpG islands is associated with silencing of genes and age-dependent methylation of several genes has been proposed as a risk factor for sporadic cancer. We examined the extent of gene methylation in pathologically normal human prostate as a function of age. EXPERIMENTAL DESIGN: We used pyrosequencing to quantitatively analyze the methylation status of nine CpG islands in normal prostate tissue DNA from 45 organ donors and 45 patients who had undergone cystoprostatectomy for bladder cancer. We also analyzed 12 pairs of matched benign and prostate cancer tissue DNA from patients with prostate cancer. RESULTS: Linear regression analysis revealed a significant increase in promoter methylation levels correlating with age for CpG islands at RARbeta2 (r = 0.4; P < 0.0001), RASSF1A (r = 0.27; P = 0.01), GSTP1 (r = 0.59; P < 0.0001), NKX2-5 (r = 0.27; P = 0.008), and ESR1 (r = 0.244; P = 0.023) in the normal prostate tissue samples studied. A calculated average methylation (z score) at all nine CpG loci analyzed in the normal prostate tissues showed a strong correlation with age (r = 0.6; P < 0.001). Comparison of the methylation level for the matched benign and prostate cancer tissues from individual patients with prostate cancer showed significantly higher methylation in the prostate cancer tissue samples for RARbeta2 (P < 0.001), RASSF1A (P = 0.005), GSTP1 (P < 0.001), NKX2-5 (P = 0.003), ESR1 (P = 0.016), and CLSTN1 (P = 0.01). CONCLUSIONS: Our findings show aberrant hypermethylation as a function of age in the normal prostate tissues. Such age-related methylation may precede and predispose to full-blown malignancy. IS - 13 JF - Clinical cancer research : an official journal of the American Association for Cancer Research SN - 1078-0432 EP - 3802 TI - Age-related DNA methylation changes in normal human prostate tissues. VL - 13 SP - 3796 AU - Kwabi-Addo, B AU - Chung, W AU - Shen, L AU - Ittmann, M AU - Wheeler, T AU - Jelinek, J AU - Issa, JP PY - 2007/07/01/ UR - http://dx.doi.org/10.1158/1078-0432.CCR-07-0085 ER -