Beta-Lactam Resistance in Salmonella Isolated from Retail Meats in the United States: National Antimicrobial Resistance Monitoring System (NARMS): 2002-2006 Export

@article{citeulike:6007697, abstract = {Ampicillin-resistant (AMPR) Salmonella (n=344) representing 32 serotypes isolated from retail meats from 2002-2006 were tested for susceptibility to 21 other antimicrobial agents, and screened for the presence of five beta-lactamase gene families (blaCMY, blaTEM, blaSHV, blaOXA, blaCTX-M) and class 1 integrons. Among the AMPR isolates, 66.9\% were resistant to [\≥]5 antimicrobials and 4.9\% to [\≥] 10 antimicrobials. Co-resistance to other beta-lactams was noted for amoxicillin/clavulanic acid (55.5\%), ceftiofur (50\%), cefoxitin (50\%), and ceftazidime (24.7\%), whereas less than 5\% of isolates were resistant to piperacillin/tazobactam (4.9\%), cefotaxime (3.5\%), ceftriaxone (2\%), and aztreonam (1.2\%). All isolates were susceptible to cefepime, imipemen and cefquinome. No Salmonella producing extended-spectrum beta-lactamases (ESBL) was found in this study. Approximately 7\% of the isolates displayed a typical MDR-AmpC phenotype, resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, tetracycline (ACSSuT), plus resistance to amoxicillin/clavulanic acid, cefoxitin, and ceftiofur with decreased susceptibility to ceftriaxone (MIC [\≥]4ug/ml). PFGE results showed that several MDR clones were geographically dispersed in different types of meats throughout the five sampling years. Additionally, 50\% of the isolates contained blaCMY, 47\% carried blaTEM-1 and 2.6\% carried both genes. Only 15\% of the isolates contained class I integrons carrying various combinations of aadA, aadB and dfrA gene cassettes. The blaCMY, blaTEM and class 1 integrons were transferable through conjugation and/or transformation. Our findings indicate a varied spectrum of co-resistance traits is present in AMPR Salmonella in the U.S. meat supply, with a continued predominance of blaCMYand blaTEM genes in beta-lactam resistant isolates. 10.1128/AEM.01158-09}, author = {Zhao, S. and Blickenstaff, K. and Glenn, A. and Ayers, S. L. and Friedman, S. L. and Abbott, J. W. and McDermott, P. F.}, citeulike-article-id = {6007697}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/AEM.01158-09}, citeulike-linkout-1 = {http://aem.asm.org/content/AEM.01158-09v1/.abstract}, citeulike-linkout-2 = {http://aem.asm.org/content/AEM.01158-09v1/.full.pdf}, day = {23}, doi = {10.1128/AEM.01158-09}, journal = {Appl. Environ. Microbiol.}, keywords = {beta-lactam-antibiotic-resistance}, month = {October}, pages = {AEM.01158-09+}, posted-at = {2009-10-25 18:37:19}, priority = {2}, title = {Beta-Lactam Resistance in Salmonella Isolated from Retail Meats in the United States: National Antimicrobial Resistance Monitoring System (NARMS): 2002-2006}, url = {http://dx.doi.org/10.1128/AEM.01158-09}, year = {2009} }

TY - JOUR ID - citeulike:6007697 L3 - citeulike-article-id:6007697 N2 - Ampicillin-resistant (AMPR) Salmonella (n=344) representing 32 serotypes isolated from retail meats from 2002-2006 were tested for susceptibility to 21 other antimicrobial agents, and screened for the presence of five beta-lactamase gene families (blaCMY, blaTEM, blaSHV, blaOXA, blaCTX-M) and class 1 integrons. Among the AMPR isolates, 66.9% were resistant to [≥]5 antimicrobials and 4.9% to [≥] 10 antimicrobials. Co-resistance to other beta-lactams was noted for amoxicillin/clavulanic acid (55.5%), ceftiofur (50%), cefoxitin (50%), and ceftazidime (24.7%), whereas less than 5% of isolates were resistant to piperacillin/tazobactam (4.9%), cefotaxime (3.5%), ceftriaxone (2%), and aztreonam (1.2%). All isolates were susceptible to cefepime, imipemen and cefquinome. No Salmonella producing extended-spectrum beta-lactamases (ESBL) was found in this study. Approximately 7% of the isolates displayed a typical MDR-AmpC phenotype, resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, tetracycline (ACSSuT), plus resistance to amoxicillin/clavulanic acid, cefoxitin, and ceftiofur with decreased susceptibility to ceftriaxone (MIC [≥]4ug/ml). PFGE results showed that several MDR clones were geographically dispersed in different types of meats throughout the five sampling years. Additionally, 50% of the isolates contained blaCMY, 47% carried blaTEM-1 and 2.6% carried both genes. Only 15% of the isolates contained class I integrons carrying various combinations of aadA, aadB and dfrA gene cassettes. The blaCMY, blaTEM and class 1 integrons were transferable through conjugation and/or transformation. Our findings indicate a varied spectrum of co-resistance traits is present in AMPR Salmonella in the U.S. meat supply, with a continued predominance of blaCMYand blaTEM genes in beta-lactam resistant isolates. 10.1128/AEM.01158-09 JF - Appl. Environ. Microbiol. TI - Beta-Lactam Resistance in Salmonella Isolated from Retail Meats in the United States: National Antimicrobial Resistance Monitoring System (NARMS): 2002-2006 SP - AEM.01158-09 KW - beta-lactam-antibiotic-resistance AU - Zhao, S AU - Blickenstaff, K AU - Glenn, A AU - Ayers, SL AU - Friedman, SL AU - Abbott, JW AU - McDermott, PF PY - 2009/10/23/ UR - http://dx.doi.org/10.1128/AEM.01158-09 ER -