Protonation of Individual Histidine Residues Is Not Required for the pH-Dependent Entry of West Nile Virus: Evaluation of the "Histidine Switch" Hypothesis Export

@article{citeulike:6072636, abstract = {Histidine residues have been hypothesized to function as sensors of environmental pH that can trigger the activity of viral fusion proteins. We investigated a requirement for histidine residues in the envelope (E) protein of West Nile virus during pH-dependent entry into cells. Each histidine was individually replaced with a nonionizable amino acid and tested functionally. In each instance, mutants capable of orchestrating pH-dependent infection were identified. These results do not support a requirement for any single histidine as a pH-sensing "switch," and they suggest that additional features of the E protein are involved in triggering pH-dependent steps in the flavivirus life cycle. 10.1128/JVI.01072-09}, author = {Nelson, Steevenson and Poddar, Subhajit and Lin, Tsai-Yu and Pierson, Theodore C.}, citeulike-article-id = {6072636}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JVI.01072-09}, citeulike-linkout-1 = {http://jvi.asm.org/content/83/23/12631.abstract}, citeulike-linkout-2 = {http://jvi.asm.org/content/83/23/12631.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19776132}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19776132}, day = {1}, doi = {10.1128/JVI.01072-09}, journal = {J. Virol.}, keywords = {his, histidine}, month = {December}, number = {23}, pages = {12631--12635}, posted-at = {2009-11-05 08:16:24}, priority = {2}, title = {Protonation of Individual Histidine Residues Is Not Required for the pH-Dependent Entry of West Nile Virus: Evaluation of the "Histidine Switch" Hypothesis}, url = {http://dx.doi.org/10.1128/JVI.01072-09}, volume = {83}, year = {2009} }

TY - JOUR ID - citeulike:6072636 L3 - citeulike-article-id:6072636 N2 - Histidine residues have been hypothesized to function as sensors of environmental pH that can trigger the activity of viral fusion proteins. We investigated a requirement for histidine residues in the envelope (E) protein of West Nile virus during pH-dependent entry into cells. Each histidine was individually replaced with a nonionizable amino acid and tested functionally. In each instance, mutants capable of orchestrating pH-dependent infection were identified. These results do not support a requirement for any single histidine as a pH-sensing "switch," and they suggest that additional features of the E protein are involved in triggering pH-dependent steps in the flavivirus life cycle. 10.1128/JVI.01072-09 IS - 23 JF - J. Virol. EP - 12635 TI - Protonation of Individual Histidine Residues Is Not Required for the pH-Dependent Entry of West Nile Virus: Evaluation of the "Histidine Switch" Hypothesis VL - 83 SP - 12631 KW - his KW - histidine AU - Nelson, Steevenson AU - Poddar, Subhajit AU - Lin, Tsai-Yu AU - Pierson, Theodore PY - 2009/12/01/ UR - http://dx.doi.org/10.1128/JVI.01072-09 ER -