Genetic reassortment of bluetongue virus serotype 11 strains in the bovine. Export

@article{citeulike:5183028, abstract = {Reassortants of bluetongue virus Serotype 11 (BTV-11) were isolated from a yearling heifer experimentally infected with two electrophoretically different strains (UC-2 and UC-8) by subcutaneous inoculation. Viruses were recovered by direct titration of sonicated blood samples onto Vero cell monolayers, which were overlaid with agarose and later plaque purified. The parental electropherotype of UC-8 was identified as the predominant virus strain during the infection; UC-2 was not isolated. UC-2 infectivity was shown by reassortants which contained genome segments that were identical in migration pattern to the parental UC-2 electropherotype. The observations demonstrate that segmental reassortment can occur during mixed infections in the bovine, between strains of the same BTV serotype.}, author = {Oberst, R. D. and Stott, J. L. and Blanchard-Channell, M. and Osburn, B. I.}, citeulike-article-id = {5183028}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/2830703}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=2830703}, issn = {0378-1135}, journal = {Veterinary microbiology}, keywords = {btv, dsrna\_virus, reassortment, serotype, vector\_borne}, month = {October}, number = {1-2}, pages = {11--18}, posted-at = {2009-07-16 17:15:11}, priority = {2}, title = {Genetic reassortment of bluetongue virus serotype 11 strains in the bovine.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2830703}, volume = {15}, year = {1987} }

TY - JOUR ID - citeulike:5183028 L3 - citeulike-article-id:5183028 N2 - Reassortants of bluetongue virus Serotype 11 (BTV-11) were isolated from a yearling heifer experimentally infected with two electrophoretically different strains (UC-2 and UC-8) by subcutaneous inoculation. Viruses were recovered by direct titration of sonicated blood samples onto Vero cell monolayers, which were overlaid with agarose and later plaque purified. The parental electropherotype of UC-8 was identified as the predominant virus strain during the infection; UC-2 was not isolated. UC-2 infectivity was shown by reassortants which contained genome segments that were identical in migration pattern to the parental UC-2 electropherotype. The observations demonstrate that segmental reassortment can occur during mixed infections in the bovine, between strains of the same BTV serotype. IS - 1-2 JF - Veterinary microbiology SN - 0378-1135 EP - 18 TI - Genetic reassortment of bluetongue virus serotype 11 strains in the bovine. VL - 15 SP - 11 KW - btv KW - dsrna_virus KW - reassortment KW - serotype KW - vector_borne AU - Oberst, RD AU - Stott, JL AU - Blanchard-Channell, M AU - Osburn, BI PY - 1987/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/2830703 ER -