Influence of glass and polymer coatings on CHO cell morphology and adhesion Export

@article{citeulike:1275409, abstract = {Successful development of cell-on-chip microsystems where living cells are deposited and grown in microfabricated structures is highly dependent on the control of cell/substrate interactions. In this study, several materials of interest were tested for CHO cell growth and morphology: (i) glass, fibronectin-, poly-l-lysine- and 3-aminopropyltriethoxysilane (APTES)--treated glass and UV/O3-modified PDMS coating on glass as well as (ii) silicon, poly-l-lysine-, APTES-, O2 plasma-treated and oxide-coated silicon. In addition, we quantitatively characterized cell adhesion to these substrates using a radial flow detachment assay. Lack of correlation between cell adhesion and cell morphology was systematically observed for all substrates. In particular, we show that PDMS coatings on glass can be finely tuned by UV/O3 treatment to enhance cell adhesion and induce elongated morphology. Moreover, we observed a low shear stress cell detachment mechanism on silicon oxide coatings on silicon wafers. It is therefore possible with these coatings to selectively influence either cell adhesion or morphology.}, author = {Sordel, Thomas and Kermarec-Marcel, Frederique and Garnier-Raveaud, Stephanie and Glade, Nicolas and Sauter-Starace, Fabien and Pudda, Catherine and Borella, Mathias and Plissonnier, Marc and Chatelain, Francois and Bruckert, Franz and Picollet-D'Hahan, Nathalie}, citeulike-article-id = {1275409}, citeulike-linkout-0 = {http://www.sciencedirect.com/science/article/B6TWB-4MG1P14-1/2/24aaedf217f161996de8f7396379e8c6}, journal = {Biomaterials}, keywords = {afm, aptes, ca, pdms}, month = {March}, number = {8}, pages = {1572--1584}, posted-at = {2007-05-03 22:20:31}, priority = {2}, title = {Influence of glass and polymer coatings on CHO cell morphology and adhesion}, url = {http://www.sciencedirect.com/science/article/B6TWB-4MG1P14-1/2/24aaedf217f161996de8f7396379e8c6}, volume = {28}, year = {2007} }

TY - JOUR ID - citeulike:1275409 L3 - citeulike-article-id:1275409 N2 - Successful development of cell-on-chip microsystems where living cells are deposited and grown in microfabricated structures is highly dependent on the control of cell/substrate interactions. In this study, several materials of interest were tested for CHO cell growth and morphology: (i) glass, fibronectin-, poly-l-lysine- and 3-aminopropyltriethoxysilane (APTES)--treated glass and UV/O3-modified PDMS coating on glass as well as (ii) silicon, poly-l-lysine-, APTES-, O2 plasma-treated and oxide-coated silicon. In addition, we quantitatively characterized cell adhesion to these substrates using a radial flow detachment assay. Lack of correlation between cell adhesion and cell morphology was systematically observed for all substrates. In particular, we show that PDMS coatings on glass can be finely tuned by UV/O3 treatment to enhance cell adhesion and induce elongated morphology. Moreover, we observed a low shear stress cell detachment mechanism on silicon oxide coatings on silicon wafers. It is therefore possible with these coatings to selectively influence either cell adhesion or morphology. IS - 8 JF - Biomaterials EP - 1584 TI - Influence of glass and polymer coatings on CHO cell morphology and adhesion VL - 28 SP - 1572 KW - afm KW - aptes KW - ca KW - pdms AU - Sordel, Thomas AU - Kermarec-Marcel, Frederique AU - Garnier-Raveaud, Stephanie AU - Glade, Nicolas AU - Sauter-Starace, Fabien AU - Pudda, Catherine AU - Borella, Mathias AU - Plissonnier, Marc AU - Chatelain, Francois AU - Bruckert, Franz AU - Picollet-D'Hahan, Nathalie PY - 2007/03// UR - http://www.sciencedirect.com/science/article/B6TWB-4MG1P14-1/2/24aaedf217f161996de8f7396379e8c6 ER -