14-3-3Sigma is required to prevent mitotic catastrophe after DNA damage. Export

@article{citeulike:383812, abstract = {14-3-3Sigma is a member of a family of proteins that regulate cellular activity by binding and sequestering phosphorylated proteins. It has been suggested that 14-3-3sigma promotes pre-mitotic cell-cycle arrest following DNA damage, and that its expression can be controlled by the p53 tumour suppressor gene. Here we describe an improved approach to the generation of human somatic-cell knockouts, which we have used to generate human colorectal cancer cells in which both 14-3-3sigma alleles are inactivated. After DNA damage, these cells initially arrested in the G2 phase of the cell cycle, but, unlike cells containing 14-3-3sigma, the 14-3-3sigma-/- cells were unable to maintain cell-cycle arrest. The 14-3-3sigma-/- cells died ('mitotic catastrophe') as they entered mitosis. This process was associated with a failure of the 14-3-3sigma-deficient cells to sequester the proteins (cyclin B1 and cdc2) that initiate mitosis and prevent them from entering the nucleus. These results may indicate a mechanism for maintaining the G2 checkpoint and preventing mitotic death.}, address = {The Johns Hopkins Oncology Center, Program in Human Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.}, author = {Chan, T. A. and Hermeking, H. and Lengauer, C. and Kinzler, K. W. and Vogelstein, B.}, citeulike-article-id = {383812}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/44188}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10524633}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10524633}, day = {7}, doi = {10.1038/44188}, issn = {0028-0836}, journal = {Nature}, keywords = {14-3-3, arrest, catastrophe, cyclinb, damage, dna, g2}, month = {October}, number = {6753}, pages = {616--620}, posted-at = {2008-05-27 22:40:11}, priority = {2}, title = {14-3-3Sigma is required to prevent mitotic catastrophe after DNA damage.}, url = {http://dx.doi.org/10.1038/44188}, volume = {401}, year = {1999} }

TY - JOUR ID - citeulike:383812 L3 - citeulike-article-id:383812 N2 - 14-3-3Sigma is a member of a family of proteins that regulate cellular activity by binding and sequestering phosphorylated proteins. It has been suggested that 14-3-3sigma promotes pre-mitotic cell-cycle arrest following DNA damage, and that its expression can be controlled by the p53 tumour suppressor gene. Here we describe an improved approach to the generation of human somatic-cell knockouts, which we have used to generate human colorectal cancer cells in which both 14-3-3sigma alleles are inactivated. After DNA damage, these cells initially arrested in the G2 phase of the cell cycle, but, unlike cells containing 14-3-3sigma, the 14-3-3sigma-/- cells were unable to maintain cell-cycle arrest. The 14-3-3sigma-/- cells died ('mitotic catastrophe') as they entered mitosis. This process was associated with a failure of the 14-3-3sigma-deficient cells to sequester the proteins (cyclin B1 and cdc2) that initiate mitosis and prevent them from entering the nucleus. These results may indicate a mechanism for maintaining the G2 checkpoint and preventing mitotic death. IS - 6753 JF - Nature SN - 0028-0836 AD - The Johns Hopkins Oncology Center, Program in Human Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA. EP - 620 TI - 14-3-3Sigma is required to prevent mitotic catastrophe after DNA damage. VL - 401 SP - 616 KW - 14-3-3 KW - arrest KW - catastrophe KW - cyclinb KW - damage KW - dna KW - g2 AU - Chan, TA AU - Hermeking, H AU - Lengauer, C AU - Kinzler, KW AU - Vogelstein, B PY - 1999/10/07/ UR - http://dx.doi.org/10.1038/44188 ER -