X-inactivation patch size in human female tissue confounds the assessment of tumor clonality. Export

@article{citeulike:3850850, abstract = {Most models of tumorigenesis assume that tumors are monoclonal in origin. This conclusion is based largely on studies using X chromosome-linked markers in females. One important factor, often ignored in such studies, is the distribution of X-inactivated cells in tissues. Because lyonization occurs early in development, many of the progeny of a single embryonic stem cell are grouped together in the adult, forming patches. As polyclonality can be demonstrated only at the borders of X-inactivation patches, the patch size is crucial in determining the chance of demonstrating polyclonality and hence the number of tumors that need to be examined to exclude polyclonality. Previously studies using X-linked genes such as glucose-6-phosphate dehydrogenase have been handicapped by the need to destroy the tissues to study the haplotypes of glucose-6-phosphate dehydrogenase [Fialkow, P.-J. (1976) Biochim. Biophys. Acta 458, 283-321] or to determine the restriction fragment length polymorphisms of X chromosome-linked genes [Vogelstein, B., Fearon, E. R., Hamilton, S. R. \& Feinberg, A. P. (1985) Science 227, 642-645]. Here we visualize X-inactivation patches in human females directly. Results show that the patch size is relatively large in both the human colon and breast, confounding assessment of tumor clonality with traditional X-inactivation studies.}, author = {Novelli, M. and Cossu, A. and Oukrif, D. and Quaglia, A. and Lakhani, S. and Poulsom, R. and Sasieni, P. and Carta, P. and Contini, M. and Pasca, A. and Palmieri, G. and Bodmer, W. and Tanda, F. and Wright, N.}, citeulike-article-id = {3850850}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0437825100}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12610207}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12610207}, day = {18}, doi = {10.1073/pnas.0437825100}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {cancer, inactivation, mutator, tumour, x}, month = {March}, number = {6}, pages = {3311--3314}, posted-at = {2009-01-05 15:33:04}, priority = {2}, title = {X-inactivation patch size in human female tissue confounds the assessment of tumor clonality.}, url = {http://dx.doi.org/10.1073/pnas.0437825100}, volume = {100}, year = {2003} }

TY - JOUR ID - citeulike:3850850 L3 - citeulike-article-id:3850850 N2 - Most models of tumorigenesis assume that tumors are monoclonal in origin. This conclusion is based largely on studies using X chromosome-linked markers in females. One important factor, often ignored in such studies, is the distribution of X-inactivated cells in tissues. Because lyonization occurs early in development, many of the progeny of a single embryonic stem cell are grouped together in the adult, forming patches. As polyclonality can be demonstrated only at the borders of X-inactivation patches, the patch size is crucial in determining the chance of demonstrating polyclonality and hence the number of tumors that need to be examined to exclude polyclonality. Previously studies using X-linked genes such as glucose-6-phosphate dehydrogenase have been handicapped by the need to destroy the tissues to study the haplotypes of glucose-6-phosphate dehydrogenase [Fialkow, P.-J. (1976) Biochim. Biophys. Acta 458, 283-321] or to determine the restriction fragment length polymorphisms of X chromosome-linked genes [Vogelstein, B., Fearon, E. R., Hamilton, S. R. & Feinberg, A. P. (1985) Science 227, 642-645]. Here we visualize X-inactivation patches in human females directly. Results show that the patch size is relatively large in both the human colon and breast, confounding assessment of tumor clonality with traditional X-inactivation studies. IS - 6 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 3314 TI - X-inactivation patch size in human female tissue confounds the assessment of tumor clonality. VL - 100 SP - 3311 KW - cancer KW - inactivation KW - mutator KW - tumour KW - x AU - Novelli, M AU - Cossu, A AU - Oukrif, D AU - Quaglia, A AU - Lakhani, S AU - Poulsom, R AU - Sasieni, P AU - Carta, P AU - Contini, M AU - Pasca, A AU - Palmieri, G AU - Bodmer, W AU - Tanda, F AU - Wright, N PY - 2003/03/18/ UR - http://dx.doi.org/10.1073/pnas.0437825100 ER -