Differential regulation of E2F transactivation by cyclin/cdk2 complexes. Export

@article{citeulike:4013783, abstract = {The mammalian transcription factor E2F plays a critical role in the expression of genes required for cellular proliferation. To understand how E2F is regulated, we have developed a reconstituted in vitro transcription assay. Using this E2F-responsive assay, we can demonstrate that E2F-mediated transcription can be directly repressed by the tumor suppressor protein pRB. This inhibition is abolished by phosphorylation of pRB with either cyclin A/cdk2 or cyclin E/cdk2. However, these cyclin/kinase complexes exhibit differences in the ability to phosphorylate E2F. Only cyclin A/cdk2 can phosphorylate E2F effectively, and this phosphorylation abolishes its ability to bind DNA and mediate trans-activation. Thus, this in vitro transcriptional assay allows activation and inactivation of E2F transcription, and our findings demonstrate how transcriptional regulation of E2F can be linked to cell cycle-dependent activation of kinases.}, author = {Dynlacht, B. D. and Flores, O. and Lees, J. A. and Harlow, E.}, citeulike-article-id = {4013783}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7958856}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7958856}, day = {1}, issn = {0890-9369}, journal = {Genes \& development}, keywords = {cdk2, cyclina, cycline, e2f}, month = {August}, number = {15}, pages = {1772--1786}, posted-at = {2009-02-05 18:56:13}, priority = {2}, title = {Differential regulation of E2F transactivation by cyclin/cdk2 complexes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7958856}, volume = {8}, year = {1994} }

TY - JOUR ID - citeulike:4013783 L3 - citeulike-article-id:4013783 N2 - The mammalian transcription factor E2F plays a critical role in the expression of genes required for cellular proliferation. To understand how E2F is regulated, we have developed a reconstituted in vitro transcription assay. Using this E2F-responsive assay, we can demonstrate that E2F-mediated transcription can be directly repressed by the tumor suppressor protein pRB. This inhibition is abolished by phosphorylation of pRB with either cyclin A/cdk2 or cyclin E/cdk2. However, these cyclin/kinase complexes exhibit differences in the ability to phosphorylate E2F. Only cyclin A/cdk2 can phosphorylate E2F effectively, and this phosphorylation abolishes its ability to bind DNA and mediate trans-activation. Thus, this in vitro transcriptional assay allows activation and inactivation of E2F transcription, and our findings demonstrate how transcriptional regulation of E2F can be linked to cell cycle-dependent activation of kinases. IS - 15 JF - Genes & development SN - 0890-9369 EP - 1786 TI - Differential regulation of E2F transactivation by cyclin/cdk2 complexes. VL - 8 SP - 1772 KW - cdk2 KW - cyclina KW - cycline KW - e2f AU - Dynlacht, BD AU - Flores, O AU - Lees, JA AU - Harlow, E PY - 1994/08/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/7958856 ER -