Inhibition of BUB1 results in genomic instability and anchorage-independent growth of normal human fibroblasts. Export

@article{citeulike:4073005, abstract = {The relative contribution of aneuploidy and gene mutations to human tumorigenesis is not yet known. Studies in mice have demonstrated that even single point mutations in oncogenes and tumor suppressor genes can dramatically increase tumor frequency. However, models to evaluate the definitive role of aneuploidy and genomic instability are not yet available. Human fibroblast cells have long been used as a tool for investigating proliferation, senescence, immortalization, and tumorigenesis, all processes that are strongly interrelated. We have now used antisense and ribozyme-mediated temporary inhibition of BUB1 to study the consequences of mitotic checkpoint failure on the development of aneuploidy. The analysis of cell colonies selected by soft agar growth showed evidence of chromosome instability and delayed senescence, without being tumorigenic in nude mice. Our data suggest that chromosomal instability and aneuploidy are early changes that precede tumorigenicity in the multistep process leading to neoplastic transformation.}, author = {Musio, A. and Montagna, C. and Zambroni, D. and Indino, E. and Barbieri, O. and Citti, L. and Villa, A. and Ried, T. and Vezzoni, P.}, citeulike-article-id = {4073005}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12782591}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12782591}, day = {1}, issn = {0008-5472}, journal = {Cancer research}, keywords = {anchorage, bub1, genome, instability}, month = {June}, number = {11}, pages = {2855--2863}, posted-at = {2009-02-20 04:03:01}, priority = {2}, title = {Inhibition of BUB1 results in genomic instability and anchorage-independent growth of normal human fibroblasts.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12782591}, volume = {63}, year = {2003} }

TY - JOUR ID - citeulike:4073005 L3 - citeulike-article-id:4073005 N2 - The relative contribution of aneuploidy and gene mutations to human tumorigenesis is not yet known. Studies in mice have demonstrated that even single point mutations in oncogenes and tumor suppressor genes can dramatically increase tumor frequency. However, models to evaluate the definitive role of aneuploidy and genomic instability are not yet available. Human fibroblast cells have long been used as a tool for investigating proliferation, senescence, immortalization, and tumorigenesis, all processes that are strongly interrelated. We have now used antisense and ribozyme-mediated temporary inhibition of BUB1 to study the consequences of mitotic checkpoint failure on the development of aneuploidy. The analysis of cell colonies selected by soft agar growth showed evidence of chromosome instability and delayed senescence, without being tumorigenic in nude mice. Our data suggest that chromosomal instability and aneuploidy are early changes that precede tumorigenicity in the multistep process leading to neoplastic transformation. IS - 11 JF - Cancer research SN - 0008-5472 EP - 2863 TI - Inhibition of BUB1 results in genomic instability and anchorage-independent growth of normal human fibroblasts. VL - 63 SP - 2855 KW - anchorage KW - bub1 KW - genome KW - instability AU - Musio, A AU - Montagna, C AU - Zambroni, D AU - Indino, E AU - Barbieri, O AU - Citti, L AU - Villa, A AU - Ried, T AU - Vezzoni, P PY - 2003/06/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/12782591 ER -