Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity. Export

@article{citeulike:4239401, abstract = {Cohesin's Smc1, Smc3, and Scc1 subunits form a tripartite ring that entraps sister DNAs. Scc3, Pds5, and Rad61 (Wapl) are regulatory subunits that control this process. We describe here smc3, scc3, pds5, and rad61 mutations that permit yeast cell proliferation and entrapment of sister DNAs by cohesin rings in the absence of Eco1, an acetyl transferase normally essential for establishing sister chromatid cohesion. The smc3 mutations cluster around and include a highly conserved lysine (K113) close to Smc3's ATP-binding pocket, which, together with K112, is acetylated by Eco1. Lethality caused by mutating both residues to arginine is suppressed by the scc3, pds5, and rad61 mutants. Scc3, Pds5, and Rad61 form a complex and inhibit entrapment of sister DNAs by a process involving the "K112/K113" surface on Smc3's ATPase. According to this model, Eco1 promotes sister DNA entrapment partly by relieving an antiestablishment activity associated with Scc3, Pds5, and Rad61.}, author = {Rowland, B. D. and Roig, M. B. and Nishino, T. and Kurze, A. and Uluocak, P. and Mishra, A. and Beckou\"{e}t, F. and Underwood, P. and Metson, J. and Imre, R. and Mechtler, K. and Katis, V. L. and Nasmyth, K.}, citeulike-article-id = {4239401}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.molcel.2009.02.028}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19328069}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19328069}, day = {27}, doi = {10.1016/j.molcel.2009.02.028}, issn = {1097-4164}, journal = {Molecular cell}, keywords = {chromatid, cohesion, smc3}, month = {March}, number = {6}, pages = {763--774}, posted-at = {2009-03-31 11:19:28}, priority = {2}, title = {Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity.}, url = {http://dx.doi.org/10.1016/j.molcel.2009.02.028}, volume = {33}, year = {2009} }

TY - JOUR ID - citeulike:4239401 L3 - citeulike-article-id:4239401 N2 - Cohesin's Smc1, Smc3, and Scc1 subunits form a tripartite ring that entraps sister DNAs. Scc3, Pds5, and Rad61 (Wapl) are regulatory subunits that control this process. We describe here smc3, scc3, pds5, and rad61 mutations that permit yeast cell proliferation and entrapment of sister DNAs by cohesin rings in the absence of Eco1, an acetyl transferase normally essential for establishing sister chromatid cohesion. The smc3 mutations cluster around and include a highly conserved lysine (K113) close to Smc3's ATP-binding pocket, which, together with K112, is acetylated by Eco1. Lethality caused by mutating both residues to arginine is suppressed by the scc3, pds5, and rad61 mutants. Scc3, Pds5, and Rad61 form a complex and inhibit entrapment of sister DNAs by a process involving the "K112/K113" surface on Smc3's ATPase. According to this model, Eco1 promotes sister DNA entrapment partly by relieving an antiestablishment activity associated with Scc3, Pds5, and Rad61. IS - 6 JF - Molecular cell SN - 1097-4164 EP - 774 TI - Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity. VL - 33 SP - 763 KW - chromatid KW - cohesion KW - smc3 AU - Rowland, BD AU - Roig, MB AU - Nishino, T AU - Kurze, A AU - Uluocak, P AU - Mishra, A AU - Beckouët, F AU - Underwood, P AU - Metson, J AU - Imre, R AU - Mechtler, K AU - Katis, VL AU - Nasmyth, K PY - 2009/03/27/ UR - http://dx.doi.org/10.1016/j.molcel.2009.02.028 ER -