Molecular cloning and expression of icarapin, a novel IgE-binding bee venom protein Export

@article{citeulike:5906828, abstract = {The 1045 bp full-length cDNA sequence of a new bee venom component was obtained by rapid amplification of cDNA ends. The 672 bp coding sequence corresponds to a protein with a signal peptide and multiple carbohydrate binding sites, and it was named icarapin. It has the new consensus sequence N-[TS]-T-S-[TV]-x-K-[VI](2)-[DN]-G-H-x-V-x-I-N-[ED]-T-x-Y-x-[DHK]-x(2,6)- [STA]-[VLFI]-x-[KR]-V-R-[VLI]-[IV]-[DN]-V-x-P. At least two transcript variants were found. Recombinant icarapin was tested for recognition by IgE antibodies and gave a positive dot blot with sera from 4 out of 5 bee venom allergic patients, all beekeepers. Indirect immunofluorescent staining localized the protein in the cuticular lining of the venom duct.}, author = {Peiren, N. and Degraaf, D. and Brunain, M. and Bridts, C. and Ebo, D. and Stevens, W. and Jacobs, F.}, citeulike-article-id = {5906828}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.febslet.2006.08.005}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0014579306009616}, day = {04}, doi = {10.1016/j.febslet.2006.08.005}, issn = {00145793}, journal = {FEBS Letters}, keywords = {bee, hymenoptera, icarapin, protein, venom}, month = {September}, number = {20}, pages = {4895--4899}, posted-at = {2009-10-07 18:45:47}, priority = {2}, title = {Molecular cloning and expression of icarapin, a novel IgE-binding bee venom protein}, url = {http://dx.doi.org/10.1016/j.febslet.2006.08.005}, volume = {580}, year = {2006} }

TY - JOUR ID - citeulike:5906828 L3 - citeulike-article-id:5906828 N2 - The 1045 bp full-length cDNA sequence of a new bee venom component was obtained by rapid amplification of cDNA ends. The 672 bp coding sequence corresponds to a protein with a signal peptide and multiple carbohydrate binding sites, and it was named icarapin. It has the new consensus sequence N-[TS]-T-S-[TV]-x-K-[VI](2)-[DN]-G-H-x-V-x-I-N-[ED]-T-x-Y-x-[DHK]-x(2,6)- [STA]-[VLFI]-x-[KR]-V-R-[VLI]-[IV]-[DN]-V-x-P. At least two transcript variants were found. Recombinant icarapin was tested for recognition by IgE antibodies and gave a positive dot blot with sera from 4 out of 5 bee venom allergic patients, all beekeepers. Indirect immunofluorescent staining localized the protein in the cuticular lining of the venom duct. IS - 20 JF - FEBS Letters SN - 00145793 EP - 4899 TI - Molecular cloning and expression of icarapin, a novel IgE-binding bee venom protein VL - 580 SP - 4895 KW - bee KW - hymenoptera KW - icarapin KW - protein KW - venom AU - Peiren, N AU - Degraaf, D AU - Brunain, M AU - Bridts, C AU - Ebo, D AU - Stevens, W AU - Jacobs, F PY - 2006/09/04/ UR - http://dx.doi.org/10.1016/j.febslet.2006.08.005 ER -