Rapid clearance of malaria circumsporozoite protein (CS) by hepatocytes. Export

@article{citeulike:2730821, abstract = {The circumsporozoite protein (CS) covers uniformly the plasma membrane of malaria sporozoites. In vitro, CS multimers bind specifically to regions of the hepatocyte plasma membrane that are exposed to circulating blood in the Disse space. The ligand is in the region II-plus of CS, an evolutionarily conserved stretch of the protein that has amino acid sequence homology to a cell adhesive motif of thrombospondin. We have now found that intravenously injected CS constructs bind rapidly to the basolateral surface of hepatocytes, provided that the recombinant proteins contain region II-plus, and that they are aggregated. Significant amounts of CS were not retained in any other organ. The striking parallelism between these in vitro and in vivo findings with the target specificity of malaria sporozoites, reinforces the hypothesis that the attachment of the parasites to hepatocytes is via region II-plus of CS.}, address = {Department of Pathology, New York University Medical Center, New York 10016.}, author = {Cerami, C. and Frevert, U. and Sinnis, P. and Takacs, B. and Nussenzweig, V.}, citeulike-article-id = {2730821}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8294876}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8294876}, day = {1}, issn = {0022-1007}, journal = {The Journal of experimental medicine}, month = {February}, number = {2}, pages = {695--701}, posted-at = {2008-04-28 20:07:36}, priority = {2}, title = {Rapid clearance of malaria circumsporozoite protein (CS) by hepatocytes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8294876}, volume = {179}, year = {1994} }

TY - JOUR ID - citeulike:2730821 L3 - citeulike-article-id:2730821 N2 - The circumsporozoite protein (CS) covers uniformly the plasma membrane of malaria sporozoites. In vitro, CS multimers bind specifically to regions of the hepatocyte plasma membrane that are exposed to circulating blood in the Disse space. The ligand is in the region II-plus of CS, an evolutionarily conserved stretch of the protein that has amino acid sequence homology to a cell adhesive motif of thrombospondin. We have now found that intravenously injected CS constructs bind rapidly to the basolateral surface of hepatocytes, provided that the recombinant proteins contain region II-plus, and that they are aggregated. Significant amounts of CS were not retained in any other organ. The striking parallelism between these in vitro and in vivo findings with the target specificity of malaria sporozoites, reinforces the hypothesis that the attachment of the parasites to hepatocytes is via region II-plus of CS. IS - 2 JF - The Journal of experimental medicine SN - 0022-1007 AD - Department of Pathology, New York University Medical Center, New York 10016. EP - 701 TI - Rapid clearance of malaria circumsporozoite protein (CS) by hepatocytes. VL - 179 SP - 695 AU - Cerami, C AU - Frevert, U AU - Sinnis, P AU - Takacs, B AU - Nussenzweig, V PY - 1994/02/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/8294876 ER -