Studies of Plasmodium falciparum cytoadherence using immortalized human brain capillary endothelial cells. Export

@article{citeulike:2911327, abstract = {The cytoadherence of Plasmodium falciparum-infected erythrocytes was studied using immortalized human brain capillary endothelial cells. The immortalized cells, denoted as BB19, derived from the human brain endothelium, were transformed with the E6E7 genes of human papilloma virus and retained their endothelial nature, i.e. tubule formation occurred with Matrigel as a substratum and the cells stained positive for Factor VIII-related antigen, or vonWillebrand's factor. Surface expression of ICAM-1, VCAM, E-selectin, and CD36 was demonstrated by immunofluorescence staining with monoclonal antibodies to these ligands. Exposure to cytokines (TNF, IFN gamma, IL-1 alpha, and IL-6) and lipopolysaccharide resulted in an increase in expression of ICAM-1, VCAM, E-selectin, and CD36. The BB19 cells bound P. falciparum-infected red blood cells with both the FCR-3 and the ITO4 strains. Antibodies to CD36 and ICAM-1 partially inhibited the binding of the FCR-3 and the ITO4 lines, respectively. These findings suggest that BB19 cells may be useful in the analysis of receptor-based cytoadherence and sequestration, as well as in the cell biology of microvessel formation.}, address = {Department of Biology, University of California, Riverside 92521, USA.}, author = {Prudhomme, J. G. and Sherman, I. W. and Land, K. M. and Moses, A. V. and Stenglein, S. and Nelson, J. A.}, citeulike-article-id = {2911327}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8875310}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8875310}, issn = {0020-7519}, journal = {International journal for parasitology}, keywords = {cells, endothelial, human}, month = {June}, number = {6}, pages = {647--655}, posted-at = {2008-06-20 19:48:53}, priority = {2}, title = {Studies of Plasmodium falciparum cytoadherence using immortalized human brain capillary endothelial cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8875310}, volume = {26}, year = {1996} }

TY - JOUR ID - citeulike:2911327 L3 - citeulike-article-id:2911327 N2 - The cytoadherence of Plasmodium falciparum-infected erythrocytes was studied using immortalized human brain capillary endothelial cells. The immortalized cells, denoted as BB19, derived from the human brain endothelium, were transformed with the E6E7 genes of human papilloma virus and retained their endothelial nature, i.e. tubule formation occurred with Matrigel as a substratum and the cells stained positive for Factor VIII-related antigen, or vonWillebrand's factor. Surface expression of ICAM-1, VCAM, E-selectin, and CD36 was demonstrated by immunofluorescence staining with monoclonal antibodies to these ligands. Exposure to cytokines (TNF, IFN gamma, IL-1 alpha, and IL-6) and lipopolysaccharide resulted in an increase in expression of ICAM-1, VCAM, E-selectin, and CD36. The BB19 cells bound P. falciparum-infected red blood cells with both the FCR-3 and the ITO4 strains. Antibodies to CD36 and ICAM-1 partially inhibited the binding of the FCR-3 and the ITO4 lines, respectively. These findings suggest that BB19 cells may be useful in the analysis of receptor-based cytoadherence and sequestration, as well as in the cell biology of microvessel formation. IS - 6 JF - International journal for parasitology SN - 0020-7519 AD - Department of Biology, University of California, Riverside 92521, USA. EP - 655 TI - Studies of Plasmodium falciparum cytoadherence using immortalized human brain capillary endothelial cells. VL - 26 SP - 647 KW - cells KW - endothelial KW - human AU - Prudhomme, JG AU - Sherman, IW AU - Land, KM AU - Moses, AV AU - Stenglein, S AU - Nelson, JA PY - 1996/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/8875310 ER -