Methods of Digital Video Microscopy for Colloidal Studies

by: John C Crocker, David G Grier

Journal of Colloid and Interface Science, Vol. 179, No. 1. (15 April 1996), pp. 298-310.

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Abstract

We describe a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions. In a typical application, these direct imaging techniques can locate submicrometer spheres to within 10 nm in the focal plane and 150 nm in depth. Combining information from a sequence of video images into single-particle trajectories makes possible measurements of quantities of fundamental and practical interest such as diffusion coefficients and pair-wise interaction potentials. The measurements we describe in detail combine the outstanding resolution of digital imaging with video-synchronized optical trapping to obtain highly accurate and reproducible results very rapidly.

@article{CrockerGrierJCollInterfSci1996, abstract = {We describe a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions. In a typical application, these direct imaging techniques can locate submicrometer spheres to within 10 nm in the focal plane and 150 nm in depth. Combining information from a sequence of video images into single-particle trajectories makes possible measurements of quantities of fundamental and practical interest such as diffusion coefficients and pair-wise interaction potentials. The measurements we describe in detail combine the outstanding resolution of digital imaging with video-synchronized optical trapping to obtain highly accurate and reproducible results very rapidly.}, author = {Crocker, John C. and Grier, David G. }, citeulike-article-id = {1304905}, doi = {http://dx.doi.org/10.1006/jcis.1996.0217}, journal = {Journal of Colloid and Interface Science}, keywords = {colloids, microscopy, particle-tracking, thesis}, month = {April}, number = {1}, pages = {298--310}, posted-at = {2007-11-02 23:40:32}, priority = {2}, title = {Methods of Digital Video Microscopy for Colloidal Studies}, url = {http://dx.doi.org/10.1006/jcis.1996.0217}, volume = {179}, year = {1996} }

RIS record

TY - JOUR ID - CrockerGrierJCollInterfSci1996 L3 - citeulike-article-id:1304905 TI - Methods of Digital Video Microscopy for Colloidal Studies JF - Journal of Colloid and Interface Science VL - 179 IS - 1 SP - 298 EP - 310 N2 - We describe a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions. In a typical application, these direct imaging techniques can locate submicrometer spheres to within 10 nm in the focal plane and 150 nm in depth. Combining information from a sequence of video images into single-particle trajectories makes possible measurements of quantities of fundamental and practical interest such as diffusion coefficients and pair-wise interaction potentials. The measurements we describe in detail combine the outstanding resolution of digital imaging with video-synchronized optical trapping to obtain highly accurate and reproducible results very rapidly. KW - colloids KW - microscopy KW - particle-tracking KW - thesis AU - Crocker, John AU - Grier, David PY - 1996/04/15/ UR - http://dx.doi.org/10.1006/jcis.1996.0217 ER -

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