Ca2+ sparks and Ca2+ waves in saponin-permeabilized rat ventricular myocytes. Export

@article{citeulike:1284430, abstract = {1. We carried out confocal Ca2+ imaging in myocytes permeabilized with saponin in 'internal' solutions containing: MgATP, EGTA and fluo-3 potassium salt. 2. Permeabilized myocytes exhibited spontaneous Ca2+ sparks and waves similar to those observed in intact myocytes loaded with fluo-3 AM. 3. In the presence of 'low' [EGTA] (0.05 mM), Ca2+ waves arose regularly, even at relatively low [Ca2+] (50-100 nM, free). Increasing [EGTA] resulted in decreased frequency and propagation velocity of Ca2+ waves. Propagating waves were completely abolished at [EGTA] > 0.3 mM. 4. The frequency of sparks increased as a function of [Ca2+] (50-400 nM range) with no sign of a high affinity Ca2+-dependent inactivation process. 5. The rate of occurrence of Ca2+ sparks was increased by calmodulin and cyclic adenosine diphosphate-ribose (cADPR).}, address = {Department of Physiology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.}, author = {Lukyanenko, V. and Gyorke, S.}, citeulike-article-id = {1284430}, citeulike-linkout-0 = {http://jp.physoc.org/cgi/content/abstract/521/3/575}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10601490}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10601490}, day = {15}, issn = {0022-3751}, journal = {J Physiol}, keywords = {ca-dependent-inactivation, ca-waves, cadpr, calmodulin, confocal, egta, fluo-3, permeabilized-myocytes, rat, spark-rate, sparks, thapsigargin, ventricular-myocytes}, month = {December}, pages = {575--585}, posted-at = {2008-07-28 02:10:20}, priority = {0}, title = {Ca2+ sparks and Ca2+ waves in saponin-permeabilized rat ventricular myocytes.}, url = {http://jp.physoc.org/cgi/content/abstract/521/3/575}, volume = {521 Pt 3}, year = {1999} }

TY - JOUR ID - citeulike:1284430 L3 - citeulike-article-id:1284430 N2 - 1. We carried out confocal Ca2+ imaging in myocytes permeabilized with saponin in 'internal' solutions containing: MgATP, EGTA and fluo-3 potassium salt. 2. Permeabilized myocytes exhibited spontaneous Ca2+ sparks and waves similar to those observed in intact myocytes loaded with fluo-3 AM. 3. In the presence of 'low' [EGTA] (0.05 mM), Ca2+ waves arose regularly, even at relatively low [Ca2+] (50-100 nM, free). Increasing [EGTA] resulted in decreased frequency and propagation velocity of Ca2+ waves. Propagating waves were completely abolished at [EGTA] > 0.3 mM. 4. The frequency of sparks increased as a function of [Ca2+] (50-400 nM range) with no sign of a high affinity Ca2+-dependent inactivation process. 5. The rate of occurrence of Ca2+ sparks was increased by calmodulin and cyclic adenosine diphosphate-ribose (cADPR). JF - J Physiol SN - 0022-3751 AD - Department of Physiology, Texas Tech University Health Sciences Center, Lubbock 79430, USA. EP - 585 TI - Ca2+ sparks and Ca2+ waves in saponin-permeabilized rat ventricular myocytes. VL - 521 Pt 3 SP - 575 KW - ca-dependent-inactivation KW - ca-waves KW - cadpr KW - calmodulin KW - confocal KW - egta KW - fluo-3 KW - permeabilized-myocytes KW - rat KW - spark-rate KW - sparks KW - thapsigargin KW - ventricular-myocytes AU - Lukyanenko, V AU - Gyorke, S PY - 1999/12/15/ UR - http://jp.physoc.org/cgi/content/abstract/521/3/575 ER -