Speeding molecular recognition by using the folding funnel: The fly-casting mechanism Export

@article{citeulike:994969, abstract = {Protein folding and binding are kindred processes. Many proteins in the cell are unfolded, so folding and function are coupled. This paper investigates how binding kinetics is influenced by the folding of a protein. We find that a relatively unstructured protein molecule can have a greater capture radius for a specific binding site than the folded state with its restricted conformational freedom. In this scenario of binding, the unfolded state binds weakly at a relatively large distance followed by folding as the protein approaches the binding site: the \^{a}€œfly-casting mechanism.\^{a}€ We illustrate this scenario with the hypothetical kinetics of binding a single repressor molecule to a DNA site and find that the binding rate can be significantly enhanced over the rate of binding of a fully folded protein.}, address = {Departments of Chemistry and Physics, University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA.}, author = {Shoemaker, Benjamin A. and Portman, John J. and Wolynes, Peter G.}, citeulike-article-id = {994969}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.160259697}, citeulike-linkout-1 = {http://www.pnas.org/content/97/16/8868.full.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/97/16/8868.full.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/10908673}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=10908673}, day = {1}, doi = {10.1073/pnas.160259697}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {casting\_wolynes, fly}, month = {August}, number = {16}, pages = {8868--8873}, posted-at = {2009-10-28 22:52:30}, priority = {2}, title = {Speeding molecular recognition by using the folding funnel: The fly-casting mechanism}, url = {http://dx.doi.org/10.1073/pnas.160259697}, volume = {97}, year = {2000} }

TY - JOUR ID - citeulike:994969 L3 - citeulike-article-id:994969 N2 - Protein folding and binding are kindred processes. Many proteins in the cell are unfolded, so folding and function are coupled. This paper investigates how binding kinetics is influenced by the folding of a protein. We find that a relatively unstructured protein molecule can have a greater capture radius for a specific binding site than the folded state with its restricted conformational freedom. In this scenario of binding, the unfolded state binds weakly at a relatively large distance followed by folding as the protein approaches the binding site: the “fly-casting mechanism.” We illustrate this scenario with the hypothetical kinetics of binding a single repressor molecule to a DNA site and find that the binding rate can be significantly enhanced over the rate of binding of a fully folded protein. IS - 16 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Departments of Chemistry and Physics, University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA. EP - 8873 TI - Speeding molecular recognition by using the folding funnel: The fly-casting mechanism VL - 97 SP - 8868 KW - casting_wolynes KW - fly AU - Shoemaker, Benjamin AU - Portman, John AU - Wolynes, Peter PY - 2000/08/01/ UR - http://dx.doi.org/10.1073/pnas.160259697 ER -